Proteomics

Dataset Information

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Chromatin transaction map during vertebrate mitotic entry

ABSTRACT: We have used a combination of chemical genetics, chromatin proteomics and imaging to map the earliest chromatin transactions during vertebrate cell entry into mitosis. Chicken DT40 Cdk1as cells undergo synchronous mitotic entry within 15 minutes following release from a 1NM-PP1-induced arrest in late G2. In addition to changes in chromatin association with nuclear pores and the nuclear envelope, earliest prophase is dominated by changes in the association of ribonucleoproteins with chromatin, particularly in the nucleolus, where pre-rRNA processing factors leave chromatin significantly before RNA polymerase I. Nuclear envelope barrier function is lost early in prophase and cytoplasmic proteins begin to accumulate on the chromatin. As a result, outer kinetochore assembly appears complete by nuclear envelope breakdown (NEBD). Most interphase chromatin proteins remain associated with chromatin until NEBD, after which their levels drop sharply.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Gallus Gallus (chicken)

SUBMITTER: Georg Kustatscher  

LAB HEAD: Juri Rappsilber

PROVIDER: PXD026385 | Pride | 2022-01-31

REPOSITORIES: Pride

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E190316_01_Quty_CS_CO_220_BE_Itaru_S191_C1.raw Raw
E190316_03_Quty_CS_CO_220_BE_Itaru_S191_C2.raw Raw
E190316_05_Quty_CS_CO_220_BE_Itaru_S191_C3.raw Raw
E190316_07_Quty_CS_CO_220_BE_Itaru_S191_C4.raw Raw
E190316_09_Quty_CS_CO_220_BE_Itaru_S191_C5.raw Raw
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Publications

Mapping the invisible chromatin transactions of prophase chromosome remodeling.

Samejima Itaru I   Spanos Christos C   Samejima Kumiko K   Rappsilber Juri J   Kustatscher Georg G   Earnshaw William C WC  

Molecular cell 20220131 3

We have used a combination of chemical genetics, chromatin proteomics, and imaging to map the earliest chromatin transactions during vertebrate cell entry into mitosis. Chicken DT40 CDK1<sup>as</sup> cells undergo synchronous mitotic entry within 15 min following release from a 1NM-PP1-induced arrest in late G<sub>2</sub>. In addition to changes in chromatin association with nuclear pores and the nuclear envelope, earliest prophase is dominated by changes in the association of ribonucleoproteins  ...[more]

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