Proteomics

Dataset Information

0

Quantitative proteome for C.elegans by 3plex mTRAQ


ABSTRACT: Vesicle-mediated transport is a fundamental part of the secretory and endocytic pathways. In addition to their role in membrane protein trafficking, Arf-like protein subfamily of small GTPases also plays an important role in development, maintenance of Golgi structure and function. Proper protein trafficking is critical for cellular integrity and studies demonstrate that aberrant protein sorting leads to various diseases in many organisms including humans. However, to date, there is no report showing the role of Golgi apparatus and Golgi proteins in organismal longevity. Organisms dynamically reprogram their transcriptome and proteome as a response to internal and external changes, which alter physiological processes including aging. Compared to many transcriptomic studies that identified aging-regulatory genes, proteomic studies that identified aging-regulatory proteins are relatively scarce. By using a quantitative proteomic approach, we identified MON-2, an Arf-Gef protein implicated in Golgi-to-endosome trafficking, as a longevity-promoting protein. We found that MON-2 is essential for the long lifespan of various longevity mutants. Our results demonstrate that Golgi-to-endosome trafficking is an integral part of lifespan regulation.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Shinyeong Ju  

LAB HEAD: Cheolju Lee

PROVIDER: PXD026411 | Pride | 2022-02-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
120929-Elegans_3plex_Offgel_00.RAW Raw
120929-Elegans_3plex_Offgel_01.RAW Raw
120929-Elegans_3plex_Offgel_02.RAW Raw
120929-Elegans_3plex_Offgel_03.RAW Raw
120929-Elegans_3plex_Offgel_04.RAW Raw
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Publications


The Golgi apparatus plays a central role in trafficking cargoes such as proteins and lipids. Defects in the Golgi apparatus lead to various diseases, but its role in organismal longevity is largely unknown. Using a quantitative proteomic approach, we found that a Golgi protein, MON-2, was up-regulated in long-lived <i>Caenorhabditis elegans</i> mutants with mitochondrial respiration defects and was required for their longevity. Similarly, we showed that DOP1/PAD-1, which acts with MON-2 to traff  ...[more]

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