Dataset Information

Yield and immunomodulatory effects of pneumococcal membrane vesicles change with bacterial growth phase

ABSTRACT: We investigated whether isolation of MVs during either the stationary-phase (24 h) or death-phase (48 h) affected their yield, immunogenicity and cytotoxicity in primary human immune cells. Death-phase vesicles showed higher yield than stationary-phase vesicles. Both vesicle types displayed excellent compatibility with primary human macrophages, and several cell lines, without cytotoxic effect. However, stationary-phase MVs showed slightly higher uptake into primary macrophages. Both vesicle types enhanced the release of inflammatory cytokines tumor necrosis factor and interleukin-6 from primary human macrophages. Proteomic analysis revealed similarities in vesicular immunogenic proteins in both vesicle types, such as pneumolysin, pneumococcal surface protein A, IgA1 protease, but stationary-phase MVs showed significantly lower autolysin levels, than death-phase MVs.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Streptococcus Pneumoniae (strain Atcc Baa-255 / R6)

SUBMITTER: Tobias Kroniger

LAB HEAD: Dörte Becher

PROVIDER: PXD026462 | Pride | 2022-02-16

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	200727_VE1_PM4_ToK_HIPS_OMV_crude_24_Rep1_F1.raw	Raw
	200727_VE1_PM4_ToK_HIPS_OMV_crude_24_Rep1_F2.raw	Raw
	200727_VE1_PM4_ToK_HIPS_OMV_crude_24_Rep1_F3.raw	Raw
	200727_VE1_PM4_ToK_HIPS_OMV_crude_24_Rep1_F4.raw	Raw
	200727_VE1_PM4_ToK_HIPS_OMV_crude_24_Rep2_F1.raw	Raw

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Publications

Yields and Immunomodulatory Effects of Pneumococcal Membrane Vesicles Differ with the Bacterial Growth Phase.

Mehanny Mina M Kroniger Tobias T Koch Marcus M Hoppstädter Jessica J Becher Dörte D Kiemer Alexandra K AK Lehr Claus-Michael CM Fuhrmann Gregor G

Advanced healthcare materials 20211112 5

Streptococcus pneumoniae infections are a leading cause of death worldwide. Bacterial membrane vesicles (MVs) are promising vaccine candidates because of the antigenic components of their parent microorganisms. Pneumococcal MVs exhibit low toxicity towards several cell lines, but their clinical translation requires a high yield and strong immunogenic effects without compromising immune cell viability. MVs are isolated during either the stationary phase (24 h) or death phase (48 h), and their yie ...[more]

PMID: 34724354

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