Dataset Information

WASH pulldown proteomics analysis

ABSTRACT: Affinity pulldown-paired quantitative proteomic analysis of HA-YFP-NLS.WASH interactors.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Tao Wang

LAB HEAD: Zhi-Hui Deng

PROVIDER: PXD026512 | Pride | 2022-02-17

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	BPI_15251_HUMAN_DENG_HV_NT_NO4.msf	Msf
	BPI_15251_HUMAN_DENG_HV_NT_NO4.raw	Raw
	checksum.txt	Txt

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Publications

WASH interacts with Ku to regulate DNA double-stranded break repair.

Wang Tao T Du Xiao-Hui XH Hong Yu Y Hong Xian X Fan Li L Zhou Jian-Wen JW Sun He H Ge Jie J Billadeau Daniel D DD Deng Zhi-Hui ZH

iScience 20211225 1

The Wiskott-Aldrich syndrome protein and SCAR homolog (WASH), an actin nucleation-promoting factor, is present in the nucleus where it regulates gene transcription and maintains nuclear organization. Here, we show that WASH interacts with core non-homologous end-joining (NHEJ) factors including Ku70/Ku80 and DNA-PKcs, and Ku70/Ku80 is involved in the recruitment of WASH to the sites of DNA double-stranded break (DSB). WASH depletion leads to increased cell sensitivity and impaired DNA repair cap ...[more]

PMID: 35036867

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