Dataset Information

Identification of the protein interaction network of STAG2 protein isoforms with inclusion and exclusion of exon32

ABSTRACT: This study involves the identification of specific interactors for STAG2ex32+ and STAG2ex32delta. Plasmids expressing YFP-STAG2 isoforms were transfected into HCT116OsTIR cells using untransfected cells as mock control. 40h post transfection, whole cell lysates were extracted and STAG2 isoforms were immunoprecipiated using GFP-TRAP. An unspecific GFP-TRAP pulldown was also conducted using lysates from untransfected cells to assess for unspecific protein binding to the beads (Mock). Significant proteins were determined by label-free quantification and statistical analysis using MaxQuant and MSstats.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Colon Cancer

SUBMITTER: Silvia Surinova

LAB HEAD: Silvia Surinova

PROVIDER: PXD041975 | Pride | 2023-05-04

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	HUMAN_SwissProt_20201106_GFP_RFP_PRCF026.fasta	Fasta
	QEP2_210813_AB_125.raw	Raw
	QEP2_210813_AB_129.raw	Raw
	QEP2_210813_AB_133.raw	Raw
	QEP2_210813_AB_137.raw	Raw

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Publications

Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading.

Porter Hayley H Li Yang Y Neguembor Maria Victoria MV Beltran Manuel M Varsally Wazeer W Martin Laura L Cornejo Manuel Tavares MT Pezić Dubravka D Bhamra Amandeep A Surinova Silvia S Jenner Richard G RG Cosma Maria Pia MP Hadjur Suzana S

eLife 20230403

Most studies of cohesin function consider the Stromalin Antigen (STAG/SA) proteins as core complex members given their ubiquitous interaction with the cohesin ring. Here, we provide functional data to support the notion that the SA subunit is not a mere passenger in this structure, but instead plays a key role in the localization of cohesin to diverse biological processes and promotes loading of the complex at these sites. We show that in cells acutely depleted for RAD21, SA proteins remain boun ...[more]

PMID: 37010886

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