Dataset Information

Fecal Transplant from Roux-Y-Gastric-Bypass rat model into a diet induced obesity rat model

ABSTRACT: Investigating alterations the intestinal microbiome in a diet induced obesity (DIO) rat model after fecal transplant from rats, which underwent Roux-Y-Gastric-Bypass surgery (RYGB). The microbiomes of the RYGB-donor rats, the DIO rats, and DIO rats after receiving the fecal transplant from the RYGB rats. As controls lean rats as well as lean, RYGB and DIO rats after antibiotics treatment were used.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

SUBMITTER: Sven Haange

LAB HEAD: Nico Jehmlich

PROVIDER: PXD026606 | Pride | 2022-08-12

REPOSITORIES: Pride

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			Action	DRS
	2020-08-11_Rat_microbiome_1.raw	Raw
	2020-08-11_Rat_microbiome_10.raw	Raw
	2020-08-11_Rat_microbiome_107.raw	Raw
	2020-08-11_Rat_microbiome_109.raw	Raw
	2020-08-11_Rat_microbiome_11.raw	Raw

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Publications

Functional changes of the gastric bypass microbiota reactivate thermogenic adipose tissue and systemic glucose control via intestinal FXR-TGR5 crosstalk in diet-induced obesity.

Münzker Julia J Haase Nadine N Till Andreas A Sucher Robert R Haange Sven-Bastiaan SB Nemetschke Linda L Gnad Thorsten T Jäger Elisabeth E Chen Jiesi J Riede Sjaak J SJ Chakaroun Rima R Massier Lucas L Kovacs Peter P Ost Mario M Rolle-Kampczyk Ulrike U Jehmlich Nico N Weiner Juliane J Heiker John T JT Klöting Nora N Seeger Gudrun G Morawski Markus M Keitel Verena V Pfeifer Alexander A von Bergen Martin M Heeren Joerg J Krügel Ute U Fenske Wiebke K WK

Microbiome 20220624 1

<h4>Background</h4>Bariatric surgery remains the most effective therapy for adiposity reduction and remission of type 2 diabetes. Although different bariatric procedures associate with pronounced shifts in the gut microbiota, their functional role in the regulation of energetic and metabolic benefits achieved with the surgery are not clear.<h4>Methods</h4>To evaluate the causal as well as the inherent therapeutic character of the surgery-altered gut microbiome in improved energy and metabolic co ...[more]

PMID: 35739571

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Project description:Analysis of gene expression profiles of epididymal fat from DIO rats; We applied a comparative functional genomics approach to evaluate diet-induced obese (DIO) rats as an obesity model Experiment Overall Design: Gene expression profiles from DIO and lean rats were generated and compared. A private colony of male Long-Evans rats was set up at Harlan (Harlan-Sprague Dawley, Indianapolis, IN). The animals had been on either a high fat/high sucrose diet (TD95217, Harlan Takled, Madison, WI) or a regular chow (Purina 5008; Ralston-Purina, St. Louis, MO) since weaning. Rats were shipped at 14-weeks of age to our facility and maintained on a 12:12-hour light-dark photoperiod (light on 10:00 PM). They were individually housed throughout the study at ambient temperature, and had free access to diets and water. After 2 weeks acclimation to the facility, body weight of the rats and weight of food consumed in the last 7 days were measured once a week. Body fat mass was analyzed by nuclear magnetic resonance (NMR) using an Echo Medical System (Houston, TX) instrument 3 times throughout the study. Fat free mass is the difference between body mass and fat mass. All animal studies were conducted in compliance with approved institutional animal care and use protocols according to NIH guidelines (NIH publication No. 86-23, 1985). OGTT was conducted after an overnight fasting when animals were 21 weeks of age. After a baseline blood collection (time 0), animals were dosed orally with 1.5 gram/kg glucose. Subsequence blood samples were collected at 30, 60, and 120 minutes post glucose challenge for the determination of plasma glucose and insulin levels. At 25 weeks of age, blood was collected by tail bleeding after overnight fasting. Rats were killed a week later at 09:00am by rapid decapitation. Trunk blood was collected and epididymal fat tissue excised. Various circulating metabolic parameters were measured under fasted (week 25) and fed conditions (week 26). Total RNA from rat epididymal fat was isolated with RNA STAT-60 (Tel-Test) according to the manufacturer's protocol. 5 micrograms of total RNA were labeled and hybridized to Affymetrix RAE230_2 arrays according to the Affymetrix protocol.