Dataset Information

Proteomic Profiling of Concurrently Isolated Primary Microvascular Endothelial Cells, Pericytes, and Smooth Muscle Cells from Adult Mouse Heart (ALK1571, PSR-OHSU)

ABSTRACT: The microcirculation serves crucial functions in adult heart that are distinct from those carried out by epicardial vessels. Microvessels are also governed by unique regulatory mechanisms, impairment of which leads to microvessel-specific pathology. While great progress has been made in understanding and treating coronary artery disease (CAD), there are few treatment options for patients with microvascular heart disease, primarily due to our limited understanding of underlying pathology. The advent of high-throughput analytical approaches of mRNA and protein expression in specific cells provides an opportunity to transform our understanding of microvessel biology and disease at the molecular level. Understanding responses of individual microvascular cells to the same physiological or pathophysiological stimuli requires the ability to isolate the specific cell types that comprise the functional units of the microcirculation in an adult heart, preferably from the same heart, to ensure that different cells have been exposed to the same in-vivo conditions. Furthermore, in-vitro functional and molecular analysis requires an integrated workflow that combines primary cell culture with high-throughput proteomic or transcriptomic analysis. Our goal was an integrated process for simultaneous isolation, culture, and proteomic profiling of the three main cell types comprising the microcirculation in adult mouse heart: endothelial cells (ECs), pericytes (PCs) and vascular smooth muscle cells (VSMCs). We developed an integrated platform for simultaneous isolation and culture of ECs, PCs and VSMCs from adult mouse heart, coupled with unbiased mass spectrometry (MS)-based characterization of protein expression in these cells. We defined microvascular cell proteomes, identified novel protein markers and confirmed established cell-specific markers. Isolating and analyzing microvascular cell types separately from the same preparation of adult mouse hearts allowed for separate investigations into the unique contributions of the different cell types to health and disease, and interactions among different cell types.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart, Pericyte Cell, Aortic Endothelial Cell, Aortic Smooth Muscle Cell

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Phillip Wilmarth

LAB HEAD: Dr. Catherine M Davis

PROVIDER: PXD026673 | Pride | 2022-06-09

REPOSITORIES: Pride

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			Action	DRS
	ALK1571_HEC1-3_HPC1-3_SMC1-3_TMT_30ug_14pctACN_25cm_120min_20141017_OT.raw	Raw
	ALK1571_HEC1-3_HPC1-3_SMC1-3_TMT_30ug_20pctACN_25cm_120min_20141017_OT.raw	Raw
	ALK1571_HEC1-3_HPC1-3_SMC1-3_TMT_30ug_22pctACN_25cm_120min_20141017_OT.raw	Raw
	ALK1571_HEC1-3_HPC1-3_SMC1-3_TMT_30ug_24pctACN_25cm_120min_20141017_OT.raw	Raw
	ALK1571_HEC1-3_HPC1-3_SMC1-3_TMT_30ug_26pctACN_25cm_120min_20141017_OT.raw	Raw

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Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.

Cao Zhiping Z Minnier Jessica J Liu Lijuan L Scott Kristin L Lyon KLL Reddy Ashok P AP Wilmarth Phillip A PA David Larry L LL Barnes Anthony P AP Grafe Marjorie R MR Kaul Sanjiv S Alkayed Nabil J NJ Davis Catherine M CM

Scientific reports 20220525 1

The microcirculation serves crucial functions in adult heart, distinct from those carried out by epicardial vessels. Microvessels are governed by unique regulatory mechanisms, impairment of which leads to microvessel-specific pathology. There are few treatment options for patients with microvascular heart disease, primarily due to limited understanding of underlying pathology. High throughput mRNA sequencing and protein expression profiling in specific cells can improve our understanding of micr ...[more]

PMID: 35614104

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