Proteomics

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Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains


ABSTRACT: Non-degradative ubiquitin chains and phosphorylation events govern signaling responses by innate immune receptors. The deubiquitinase CYLD in complex with SPATA2 is recruited to receptor signaling complexes by the ubiquitin ligase LUBAC and regulates Met1- and Lys63-linked polyubiquitin and receptor signaling outcomes. Here, we investigated the molecular determinants of CYLD activity. We reveal that two CAP-Gly domains in CYLD are ubiquitin binding domains and demonstrate a requirement of CAP-Gly3 for CYLD activity and regulation of immune receptor signaling. Moreover, we identify a phosphorylation switch outside of the catalytic USP domain, which activates CYLD selectively towards Lys63-linked polyubiquitin. The phosphorylated residue Ser568 is a novel TNF-regulated phosphorylation site in CYLD and works in concert with Ser418 to enable CYLD-mediated deubiquitination and immune receptor signaling. We propose that phosphorylated CYLD together with SPATA2 and LUBAC functions as a ubiquitin-editing complex that rebalances Lys63- and Met1-linked polyubiquitin at receptor signaling complexes, resulting in escalated LUBAC signaling.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Georgina Berridge  

LAB HEAD: Roman Fischer

PROVIDER: PXD026791 | Pride | 2021-11-02

REPOSITORIES: Pride

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Publications

Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains.

Elliott Paul R PR   Leske Derek D   Wagstaff Jane J   Schlicher Lisa L   Berridge Georgina G   Maslen Sarah S   Timmermann Frederik F   Ma Biao B   Fischer Roman R   Freund Stefan M V SMV   Komander David D   Gyrd-Hansen Mads M  

Cell reports 20211001 1


Non-degradative ubiquitin chains and phosphorylation events govern signaling responses by innate immune receptors. The deubiquitinase CYLD in complex with SPATA2 is recruited to receptor signaling complexes by the ubiquitin ligase LUBAC and regulates Met1- and Lys63-linked polyubiquitin and receptor signaling outcomes. Here, we investigate the molecular determinants of CYLD activity. We reveal that two CAP-Gly domains in CYLD are ubiquitin-binding domains and demonstrate a requirement of CAP-Gly  ...[more]

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