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SARS_CoV-2 substrate profiling in interferon-treated BEAS-2B cell proteomes

ABSTRACT: To profile the human substrate degradome of the SARS-CoV-2 main viral protease and to investigate whether 3CLpro cleavage of cellular substrates dampens host antiviral responses induced by type I interferons, we treated BEAS-2B cells with interferon-alpha (N = 3), interferon-beta (N = 3), or vehicle (N = 3), retrieve and exposed native proteome extracts from human embryonic kidney to 3CLpro cleavage. Then, N Terminal Amine Labeling of Substates (TAILS) was used to identify the substrates. Here, heavy [+34] isotope dimethyl labeling is performed at the whole protein level in the 3CLpro-exposed samples, and the P1' side of substrates substrates was revealed by comparing to the samples exposed to inactive mutant labeled with ligh [+28 Da] dimethyl.

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Yoan Machado

LAB HEAD: Christopher M. Overall

PROVIDER: PXD026815 | Pride | 2021-11-03

REPOSITORIES: Pride

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			Action	DRS
	2000509_IP_YM_TAILS1_ACN_C1_01_12130.d.zip	Other
	2000509_IP_YM_TAILS1_ACN_C1_01_12130.mgf.mgf	Mgf
	2000509_IP_YM_TAILS1_ACN_C1_01_12130.mgf.mzid.gz	Mzid
	2000509_IP_YM_TAILS1_MeOH_C1_01_12112.d.zip	Other
	2000509_IP_YM_TAILS1_MeOH_C1_01_12112.mgf.mgf	Mgf

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