Proteomics

Dataset Information

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Rat podocyte degradomics - N-Degradomic Analysis Reveals a Proteolytic Network Processing the Podocyte Cytoskeleton

ABSTRACT: Regulated intracellular proteolysis is essential in maintaining the integrity of podocytes and the glomerular filtration barrier of the kidney. Altered proteolytic substrate turnover has been associated with various glomerular diseases ranging from diabetic nephropathy to focal and segmental glomerulosclerosis. However, thus far it has not been possible to systematically identify proteolytically cleaved proteins although some of the proteases have been characterized. Here we applied TAILS to identify N-termini in rat glomeruli and their changes on PAN-induced injury.

INSTRUMENT(S): impact II

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Kidney

SUBMITTER: Pitter Huesgen  

LAB HEAD: Pitter Huesgen

PROVIDER: PXD006356 | Pride | 2017-07-20

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
170329NW0007TAILS_GH7_01_2044.d.zip Other
170329NW0008TAILS_GH8_01_2046.d.zip Other
170329NW0009TAILS_GH9_01_2047.d.zip Other
170329NW0010TAILS_GH10_01_2048.d.zip Other
170331NW0007TAILSdiluted_GH7_01_2056.d.zip Other
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Publications

Regulated intracellular proteostasis, controlled in part by proteolysis, is essential in maintaining the integrity of podocytes and the glomerular filtration barrier of the kidney. We applied a novel proteomics technology that enables proteome-wide identification, mapping, and quantification of protein N-termini to comprehensively characterize cleaved podocyte proteins in the glomerulus <i>in vivo</i> We found evidence that defined proteolytic cleavage results in various proteoforms of important  ...[more]

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