Dataset Information

MiR141 Proteomic profile in Ovarian Cancer

ABSTRACT: Over the last two decades, mounting evidence has demonstrated the aberrant expression of miRNAs in different human malignancies, including ovarian carcinomas. In this study, we found that miR-141 were secreted by ovarian cancer cells and transferred to the stromal cells by exosomal pathway. To clarify the mechanisms that miR-141 remodeling stromal fibroblast and reprogramming the relevant signals, miR-141 were overexpressed in WPMY-1 and their respective scrambled control were send for proteomic analysis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stromal Cell Of Ovarian Cortex

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Xueyu WANG

LAB HEAD: Dr. David W. CHAN

PROVIDER: PXD026929 | Pride | 2023-03-11

REPOSITORIES: Pride

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Publications

Tumor-secreted exosomal miR-141 activates tumor-stroma interactions and controls premetastatic niche formation in ovarian cancer metastasis.

Mo Yulan Y Leung Leanne L LL Mak Celia S L CSL Wang Xueyu X Chan Wai-Sun WS Hui Lynn M N LMN Tang Hermit W M HWM Siu Michelle K Y MKY Sharma Rakesh R Xu Dakang D Tsui Stephen K W SKW Ngan Hextan Y S HYS Yung Mingo M H MMH Chan Karen K L KKL Chan David W DW

Molecular cancer 20230109 1

<h4>Background</h4>Metastatic colonization is one of the critical steps in tumor metastasis. A pre-metastatic niche is required for metastatic colonization and is determined by tumor-stroma interactions, yet the mechanistic underpinnings remain incompletely understood.<h4>Methods</h4>PCR-based miRNome profiling, qPCR, immunofluorescent analyses evaluated the expression of exosomal miR-141 and cell-to-cell communication. LC-MS/MS proteomic profiling and Dual-Luciferase analyses identified YAP1 as ...[more]

PMID: 36624516

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			Action	DRS
	WPMY_1_Scramble_LFQ1.raw	Raw
	WPMY_1_Scramble_LFQ2.raw	Raw
	WPMY_1_Scramble_LFQ3.raw	Raw
	WPMY_1_miR142_LFQ1.raw	Raw
	WPMY_1_miR142_LFQ2.raw	Raw