Dataset Information

O-glycoproteome of Tannerella forsythia

ABSTRACT: Tannerella forsythia is a Gram-negative oral pathogen known to possess an O-glycosylation system responsible for targeting multiple proteins associated with virulence at the three-residue motif (D)(S/T)(A/I/L/V/M/T). Multiple proteins have been identified to be decorated with a decasaccharide glycan composed of a poorly defined core plus a species-specific portion whose biosynthesis is largely characterized. To date, the glycosylation of mainly the two S-layer glycoproteins, TfsA and TfsB has been studied yet the true extent of glycosylation within this species has not been explored. In the present study we explore the glycoproteome of T. forsythia employing FAIMS based glycopeptide enrichment of a cell membrane fraction. We demonstrate that at least 13 glycans are utilized within the T. forsythia glycoproteome varying with respect to the presence of the three terminal sugars and the presence of fucose and digitoxose branches at the reducing end. To improve the localization of glycosylation events and enhance the detection of glycopeptides we applied trifluoromethanesulfonic acid treatment to allow the selective chemical cleavage of glycans. By reducing the chemical complexity of glycopeptides this dramatically improved the number of glycopeptides identified and our ability to localize glycosylation sites by ETD fragmentation leading to the identification of 312 putative glycosylation sites in 145 glycoproteins. Glycosylation site analysis revealed that glycosylation occurs on a much broader glycosylation motif than initially reported with glycosylation found at (D)(S/T)(A/I/L/V/M/T/S/C/G/F). The data confirm earlier predictions of hundreds of possible O-glycoproteins present in this organism.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Tannerella Forsythia

SUBMITTER: Paul Veith

LAB HEAD: Eric Reynolds

PROVIDER: PXD026989 | Pride | 2021-10-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	200928_PaulV_PV-1152_TFM_1.raw	Raw
	200928_PaulV_PV-1152_TFM_10.raw	Raw
	200928_PaulV_PV-1152_TFM_11.raw	Raw
	200928_PaulV_PV-1152_TFM_12.raw	Raw
	200928_PaulV_PV-1152_TFM_13.raw	Raw

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Publications

Characterization of the O-Glycoproteome of Tannerella forsythia.

Veith Paul D PD Scott Nichollas E NE Reynolds Eric C EC

mSphere 20210915 5

Tannerella forsythia is a Gram-negative oral pathogen known to possess an O-glycosylation system responsible for targeting multiple proteins associated with virulence at the three-residue motif (D)(S/T)(A/I/L/V/M/T). Multiple proteins have been identified to be decorated with a decasaccharide glycan composed of a poorly defined core plus a partially characterized species-specific section. To date, glycosylation studies have focused mainly on the two S-layer glycoproteins, TfsA and TfsB, so the t ...[more]

PMID: 34523981

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