Dataset Information

Ultrasmall epiparasitic Saccharibacteria suppresses gingival inflammation and bone loss through host bacterial modulation

ABSTRACT: Saccharibacteria (TM7) are obligate epibionts living on the surface of their host bacteria, and strongly correlated with dysbiotic microbiomes during periodontitis and other inflammatory diseases, suggesting they are putative pathogens. However, due to the recalcitrance of TM7 cultivation, no causal research has been conducted to investigate their role in inflammatory diseases. Here, we isolated multiple TM7 species on their host bacteria from periodontitis patients. These TM7 species reduced inflammation and consequential bone loss by modulating their host bacterial pathogenicity in mouse ligature-induced periodontitis model. Two host bacterial functions involved in collagen binding and utilization of eukaryotic sialic acid were identified as required for inducing bone loss and altered by TM7 association. This down-regulation of host bacterial pathogenicity by TM7 was shown for multiple TM7/host bacteria pairs, suggesting that, in contrast to their suspected pathogenic role, TM7 could protect mammalian hosts from inflammatory damage induced by their host bacteria.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Schaalia Odontolytica

TISSUE(S): Cell Suspension Culture, Biological Regulation

DISEASE(S): Periodontitis

SUBMITTER: Fabian Schulte

LAB HEAD: Batileg Bor

PROVIDER: PXD026999 | Pride | 2023-03-11

REPOSITORIES: Pride

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			Action	DRS
	20200731_bat_cellwall_proteome_ac_reg1_10.mgf	Mgf
	20200731_bat_cellwall_proteome_ac_reg1_10.mzxml	Mzxml
	20200731_bat_cellwall_proteome_ac_reg1_10.raw	Raw
	20200731_bat_cellwall_proteome_ac_reg1_6.mgf	Mgf
	20200731_bat_cellwall_proteome_ac_reg1_6.mzxml	Mzxml

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Publications

Episymbiotic Saccharibacteria suppresses gingival inflammation and bone loss in mice through host bacterial modulation.

Chipashvili Otari O Utter Daniel R DR Bedree Joseph K JK Ma Yansong Y Schulte Fabian F Mascarin Gabrielle G Alayyoubi Yasmin Y Chouhan Deepak D Hardt Markus M Bidlack Felicitas F Hasturk Hatice H He Xuesong X McLean Jeffrey S JS Bor Batbileg B

Cell host & microbe 20211011 11

Saccharibacteria (TM7) are obligate epibionts living on the surface of their host bacteria and are strongly correlated with dysbiotic microbiomes during periodontitis and other inflammatory diseases, suggesting they are putative pathogens. However, due to the recalcitrance of TM7 cultivation, causal research to investigate their role in inflammatory diseases is lacking. Here, we isolated multiple TM7 species on their host bacteria from periodontitis patients. These TM7 species reduce inflammatio ...[more]

PMID: 34637779

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