Dataset Information

FIRST ATTEMPT TO COUPLE PROTEOMIC WITH AhR REPORTER GENE BIOASSAY IN SOIL POLLUTION MONITORING AND ASSESSMENT

ABSTRACT: A topsoil sample obtained from a highly industrialized area was tested on CALUX® cell line and exposed cells processed with proteomic and bioinformatics analyses. GC-MS/MS analysis of topsoil extracts was also performed to confirm PCDD, PCDF and dl-PCBs presence. CALUX® and GC-MS/MS data confirmed the presence of PCDD/Fs and dl-PCBs in the topsoil extract. Proteomic analysis of the cells exposed to the soil extracts identified 43 differential proteins. Enrichment analysis highlights biological processes such as cellular response to chemical stimulus, to stress, response to inorganic substance, regulation of translation, regulation of apoptotic process as well as response to organonitrogen compounds in light of particular drugs and compounds, extrapolated by bioinformatics and linked to the identified proteins modifications. These results confirm the complex epidemiological situation occurring among Taranto inhabitants and underline the need to further investigate the presence and sources of inferred chemicals in Taranto soils. The combined analysis reveals a more complex scenarios of chemicals able to affect cellular pathways and leading to toxicities rather than those identified by the bioassay. The approach to couple proteomic to CALUX® is a promising tool and deserves further investigation to be applied in soil pollution monitoring and risk assessment.

INSTRUMENT(S): autoflex

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture

SUBMITTER: Enxhi Shaba

LAB HEAD: Prof. Luca Bini

PROVIDER: PXD027074 | Pride | 2022-02-17

REPOSITORIES: Pride

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First Attempt to Couple Proteomics with the <i>AhR</i> Reporter Gene Bioassay in Soil Pollution Monitoring and Assessment.

Landi Claudia C Liberatori Giulia G Cotugno Pietro P Sturba Lucrezia L Vannuccini Maria Luisa ML Massari Federica F Miniero Daniela Valeria DV Tursi Angelo A Shaba Enxhi E Behnisch Peter A PA Carleo Alfonso A Di Giuseppe Fabrizio F Angelucci Stefania S Bini Luca L Corsi Ilaria I

Toxics 20211229 1

A topsoil sample obtained from a highly industrialized area (Taranto, Italy) was tested on the DR-CALUX<sup>®</sup> cell line and the exposed cells processed with proteomic and bioinformatics analyses. The presence of polyhalogenated compounds in the topsoil extracts was confirmed by GC-MS/MS analysis. Proteomic analysis of the cells exposed to the topsoil extracts identified 43 differential proteins. Enrichment analysis highlighted biological processes, such as the cellular response to a chemic ...[more]

PMID: 35051051

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