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Evidence of immune modulators in the secretome of the equine tapeworm Anoplocephala perfoliata

ABSTRACT: Anoplocephala perfoliata is a neglected gastro-intestinal tapeworm, commonly infecting horses worldwide. Molecular investigation of A. perfoliata is hampered by a lack of tools to better un-derstand the host-parasite interface. This interface is likely influenced by parasite derived immune modulators released in the secretome as free proteins or components of extracellular vesicles (EVs). Therefore, adult RNA was sequenced and de novo assembled to generate the first A. perfoliata transcriptome. In addition, excretory secretory products (ESP) from adult A. perfoliata were col-lected and EVs isolated using size exclusion chromatography, prior to proteomic analysis of the EVs, the EV surface and EV depleted ESP. Transcriptome analysis revealed 454 sequences ho-mologous to known helminth immune modulators including 2 novel Sigma class GSTs, 5 α-HSP90s and 3 α-enolases with isoforms of all three observed within the proteomic analysis of the secretome. Furthermore, secretome proteomics identified common helminth proteins across each sample with known EV markers, such as annexins and tetraspanins, observed in EV fractions. Importantly, 49 of the 454 putative immune modulators were identified across the secretome proteomics contained within and on the surface of EVs in addition to those identified in free ESP. This work provides the molecular tools for A. perfoliata to reveal key players in the host-parasite interaction within the horse host.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Anoplocephala Perfoliata

TISSUE(S): Extracellular Vesicle

DISEASE(S): Parasitic Infectious Disease

SUBMITTER: Russ Morphew

LAB HEAD: Russell M Morphew

PROVIDER: PXD027105 | Pride | 2021-07-26

REPOSITORIES: Pride

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			Action	DRS
	210320_RM_MSEV_3-1.mgf	Mgf
	210320_RM_MSEV_3-1.raw	Raw
	210320_RM_MSEV_3-2.mgf	Mgf
	210320_RM_MSEV_3-2.raw	Raw
	210320_RM_MSEV_3-3.mgf	Mgf

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