Proteomics

Dataset Information

0

Search for the interactors of Arabidopsis thaliana myosin receptor MRF7


ABSTRACT: This project aims at identifying the binding partners of the Arabidopsis thaliana MRF7 protein and of its truncated form ΔMRF7, which lacks a potential protein binding domain DUF593 at the N-terminus. A.thaliana plants stably overexpressing GFP-MRF7 and GFP-ΔMRF7 were generated by floral dipping of Col-0 plants. Co-immunoprecipitation was carried out using the GFP-Trap®_A beads technology (Chromotek) and samples thus obtained were analysed through Liquid Chromatography-Mass Spectrometry

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Root, Shoot

SUBMITTER: Chiara Perico  

LAB HEAD: Imogen Sparkes

PROVIDER: PXD027155 | Pride | 2022-02-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Col_0_WT_Repeat1.mgf Mgf
Col_0_WT_Repeat1.msf Msf
Col_0_WT_Repeat1.raw Raw
GFP_Delta_MRF7_Repeat1.mgf Mgf
GFP_Delta_MRF7_Repeat1.msf Msf
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Publications

Arabidopsis thaliana myosin XIK is recruited to the Golgi through interaction with a MyoB receptor.

Perico Chiara C   Gao Hongbo H   Heesom Kate J KJ   Botchway Stanley W SW   Sparkes Imogen A IA  

Communications biology 20211013 1


Plant cell organelles are highly mobile and their positioning play key roles in plant growth, development and responses to changing environmental conditions. Movement is acto-myosin dependent. Despite controlling the dynamics of several organelles, myosin and myosin receptors identified so far in Arabidopsis thaliana generally do not localise to the organelles whose movement they control, raising the issue of how specificity is determined. Here we show that a MyoB myosin receptor, MRF7, specific  ...[more]

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