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Transcriptome of a malO deletion mutant of Clostridium perfringens during stationary phase growth.

ABSTRACT: The MalNO is a putative two-component signal transduction system, previously known as the VirJI sytem. MalO is the putative cognate response regulator of the MalN sensor histidine kinase. Based on previous evidence that suggested the plc gene, encoding ?-toxin, was upregulated during stationary phase in the malO mutant, microarrays were used to analyse the transcriptome of a malO mutant during stationary phase growth. Total RNA was isolated from stationary phase cells of the malO mutant and the wild-type control. Gene expression levels were compared between the malO mutant and wild-type strain 13

ORGANISM(S): Clostridium perfringens str. 13

SUBMITTER: Julian Rood

PROVIDER: E-GEOD-28699 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of a two-component signal transduction system that regulates maltose genes in Clostridium perfringens.

Hiscox Thomas J TJ Ohtani Kaori K Shimizu Tohru T Cheung Jackie K JK Rood Julian I JI

Anaerobe 20140823

Clostridium perfringens is a Gram-positive rod that is widely distributed in nature and is the etiological agent of several human and animal diseases. The complete genome sequence of C. perfringens strain 13 has been determined and multiple two-component signal transduction systems identified. One of these systems, designated here as the MalNO system, was analyzed in this study. Microarray analysis was used to carry out functional analysis of a malO mutant. The results, which were confirmed by q ...[more]

PMID: 25152227

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