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ITRAQ-based quantitative proteomic analysis of nuclear proteome in VPS35-depleted HeLa cells

ABSTRACT: Stable VPS35 knockdown HeLa cells were generated using lentiviral short hairpin RNA (shRNA) construct. Isobaric tags for relative and absolute quantitation (iTRAQ)-based mass spectrometry was used to measure the changes of nuclear protein abundance in VPS35-depleted HeLa cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Zhi-Hui Deng

LAB HEAD: Zhi-Hui Deng

PROVIDER: PXD027166 | Pride | 2022-08-12

REPOSITORIES: Pride

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			Action	DRS
	BPI_16383_xibaohe_itraq_1-8-uniprot-human.msf	Msf
	BPI_16383_xibaohe_itraq_1.raw	Raw
	BPI_16383_xibaohe_itraq_10.raw	Raw
	BPI_16383_xibaohe_itraq_11.raw	Raw
	BPI_16383_xibaohe_itraq_12.raw	Raw

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Publications

ITRAQ-based quantitative proteomic analysis reveals that VPS35 promotes the expression of MCM2-7 genes in HeLa cells.

Hong Xian X Wang Tao T Du Juan J Hong Yu Y Yang Cai-Ping CP Xiao Wei W Li Yang Y Wang Ming M Sun He H Deng Zhi-Hui ZH

Scientific reports 20220611 1

Vacuolar protein sorting 35 (VPS35) is a major component of the retromer complex that regulates endosomal trafficking in eukaryotic cells. Recent studies have shown that VPS35 promotes tumor cell proliferation and affects the nuclear accumulation of its interacting partner. In this study, isobaric tags for relative and absolute quantitation (iTRAQ)-based mass spectrometry were used to measure the changes in nuclear protein abundance in VPS35-depleted HeLa cells. A total of 47 differentially expr ...[more]

PMID: 35690672

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