Dataset Information

GFP-DLC1 interactome in MCF7 breast cancer cells

ABSTRACT: The Rho GTPase activating protein Deleted in Liver Cancer 1 (DLC1) is frequently downregulated through genetic and epigenetic mechanisms in various malignancies, leading to aberrant Rho GTPase signaling and thus facilitating cancer progression. The aim of this project was to identify novel interaction partners, that could be involved in the regulation of DLC1 proteasomal degradation.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Breast

DISEASE(S): Breast Cancer

SUBMITTER: Nicolas Nalpas

LAB HEAD: Monilola Olayioye

PROVIDER: PXD027186 | Pride | 2022-05-20

REPOSITORIES: Pride

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			Action	DRS
	20170529_CO_0698MoOl_R01.raw	Raw
	20170529_CO_0698MoOl_R02.raw	Raw
	search.zip	Other

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Publications

Proteasomal turnover of the RhoGAP tumor suppressor DLC1 is regulated by HECTD1 and USP7.

Frey Yannick Y Franz-Wachtel Mirita M Macek Boris B Olayioye Monilola A MA

Scientific reports 20220323 1

The Rho GTPase activating protein Deleted in Liver Cancer 1 (DLC1) is frequently downregulated through genetic and epigenetic mechanisms in various malignancies, leading to aberrant Rho GTPase signaling and thus facilitating cancer progression. Here we show that in breast cancer cells, dysregulation of DLC1 expression occurs at the protein level through rapid degradation via the ubiquitin-proteasome system. Using mass spectrometry, we identify two novel DLC1 interaction partners, the ubiquitin-l ...[more]

PMID: 35322810

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