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Proteomic effects of lysergic acid diethylamide in iPSC-derived brain organoids

ABSTRACT: Lysergic acid diethylamide (LSD), considered the prototypical psychedelic molecule, has effects on consciousness and perception. However, the molecular mechanisms involved in LSD effects in human neural cells and how they may be translated into changes in physiology are still poorly understood. Here we used human cerebral organoids as a biological live model to explore the response of human brain cells to LSD. Mass spectrometry-based proteomics was performed to gain insights on molecular targets and signaling pathways involved in the mid and long-term effects of LSD.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Juliana Minardi Nascimento

LAB HEAD: Daniel Martins-de-Souza

PROVIDER: PXD027369 | Pride | 2024-10-17

REPOSITORIES: Pride

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			Action	DRS
	Organoid_LSD1.rar	Other
	Organoids_LSD_peptideIon.csv	Csv
	Organoids_LSD_peptides.csv	Csv
	Organoids_LSD_poteins.csv	Csv

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Publications

LSD Modulates Proteins Involved in Cell Proteostasis, Energy Metabolism and Neuroplasticity in Human Cerebral Organoids.

N Costa Marcelo M Goto-Silva Livia L M Nascimento Juliana J Domith Ivan I Karmirian Karina K Feilding Amanda A Trindade Pablo P Martins-de-Souza Daniel D K Rehen Stevens S

ACS omega 20240816 34

Proteomic analysis of human cerebral organoids may reveal how psychedelics regulate biological processes, shedding light on drug-induced changes in the brain. This study elucidates the proteomic alterations induced by lysergic acid diethylamide (LSD) in human cerebral organoids. By employing high-resolution mass spectrometry-based proteomics, we quantitatively analyzed the differential abundance of proteins in cerebral organoids exposed to LSD. Our findings indicate changes in proteostasis, ener ...[more]

PMID: 39220485

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