Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

TNF-alpha stimulated secretome of schizophrenia-sourced iPSC-derived astrocytes

ABSTRACT: Schizophrenia is a complex and severe neuropsychiatric disorder, with a wide range of debilitating symptoms. Several aspects of its multifactorial complexity are still unknown, and some are accepted to be an early developmental deficiency with a more specifically neurodevelopmental origin. ??Molecular and functional abnormalities in astrocytes play a role in the etiology and pathogenesis of schizophrenia. In this study, human induced pluripotent stem cells (hiPSC)-derived astrocytes from schizophrenia patients were investigated regarding their proteome, inflammatory responses, and their secretome effect on vascularization. Proteomic analysis revealed alterations in proteins related to immune function and vascularization. Taken together, our results suggest that schizophrenia astrocytes are immunologically dysfunctional which might affect vascularization through secreted factors.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Juliana Minardi Nascimento

LAB HEAD: Daniel Martins-de-Souza

PROVIDER: PXD031650 | Pride | 2023-04-27

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Astrocytes_CondMed_SCZ_PeptideIon.csv	Csv
	Astrocytes_CondMed_SCZ_Peptides.csv	Csv
	Astrocytes_CondMed_SCZ_Proteins.csv	Csv
	Astrocytes_SCZ_secretome.rar	Other

Items per page:

1 - 4 of 4

Similar Datasets

Proteomic signatures of schizophrenia-sourced iPSC-derived astrocytes

2023-04-27 | PXD031640 | Pride

Proteomic signatures of schizophrenia-sourced iPSC-derived brain organoids

Project description:Schizophrenia is a complex and severe neuropsychiatric disorder, with a wide range of debilitating symptoms. Several aspects of its multifactorial complexity are still unknown, and some are accepted to be an early developmental deficiency with a more specifically neurodevelopmental origin. Understanding timepoints of disturbances during neural cell differentiation processes could lead to an insight into the development of the disorder. In this context, human brain organoids and neural cells differentiated from patient-derived induced pluripotent stem cells are of great interest as a model to study the developmental origins of the disease. Here we evaluated the differential expression of proteins of schizophrenia patient-derived neural progenitors, early neurons, and brain organoids. Using bottom-up shotgun proteomics with a label-free approach for quantitative analysis. Multiple dysregulated proteins were found in pathways related to synapses, in line with postmortem tissue studies of schizophrenia patients. However, organoids and immature neurons exhibit impairments in pathways never before found in patient-derived induced pluripotent stem cell studies, such as spliceosomes and amino acid metabolism. In conclusion, here we provide comprehensive, large-scale, protein-level data that may uncover underlying mechanisms of the developmental origins of schizophrenia.

2023-03-11 | PXD026381 | Pride

Proteomic signatures of schizophrenia-sourced iPSC-derived neural progenitors and neurons

2023-03-11 | PXD026593 | Pride

Lysergic acid dethylamide proteomics modulation in brain organoids

Project description:Lysergic acid diethylamide (LSD), considered the prototypical psychedelic molecule, has effects on consciousness and perception. However, the molecular mechanisms involved in LSD effects in human neural cells and how they may be translated into changes in physiology are still poorly understood. Here we used human cerebral organoids as a biological live model to explore the response of human brain cells to LSD. Mass spectrometry-based proteomics was performed to gain insights on molecular targets and signaling pathways involved in the mid and long-term effects of LSD.

2024-10-17 | PXD037814 | Pride

Proteomic effects of lysergic acid diethylamide in iPSC-derived brain organoids

2024-10-17 | PXD027369 | Pride

Human neurospheres ZIKV infection

Project description:Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular consequences of ZIKV to human brain development are still not fully understood. Here we describe alterations in human neurospheres derived from induced pluripotent stem (iPS) cells infected with the strain of Zika virus that is circulating in Brazil. Combining proteomics and mRNA transcriptional profiling, over 500 proteins and genes associated with the Brazilian ZIKV infection were found to be differentially expressed. These genes and proteins provide an interactome map, which indicates that ZIKV controls the expression of RNA processing bodies, miRNA biogenesis and splicing factors required for self-replication. It also suggests that impairments in the molecular pathways underpinning cell cycle and neuronal differentiation are caused by ZIKV. These results point to biological mechanisms implicated in brain malformations, which are important to further the understanding of ZIKV infection and can be exploited as therapeutic potential targets to mitigate it.

2018-08-29 | PXD010866 | Pride

ZIKV infection of human neurospheres

2018-10-27 | PXD004588 | Pride

Proteomic changes of ZIKV and DENV in iPS-derived neurospheres

Project description:Brain abnormalities and congenital malformations have been linked to the circulating strain of Zika virus (ZIKV) in Brazil since 2016 during the microcephaly outbreak; however, the molecular mechanisms behind several of these alterations and differential viral molecular targets have not been fully elucidated. Here we explore the proteomic alterations induced by ZIKV by comparing the Brazilian (Br ZIKV) and the African (MR766) viral strains, in addition to comparing them to the molecular responses to the Dengue virus (DENV). Neural stem cells (NSCs) derived from induced pluripotent stem (iPSCs) were cultured both as monolayers and in suspension (resulting in neurospheres), which were then infected with ZIKV (Br ZIKV or ZIKV MR766) or DENV to assess alterations within neural cells. Large-scale proteomic analyses allowed the comparison not only between viral strains but also regarding the two- and three-dimensional cellular models of neural cells derived from iPSCs, and the effects on their interaction. Altered pathways and biological processes were observed, related to cell death, cell cycle dysregulation, and neurogenesis. These results reinforce already published data and provide further information regarding the biological alterations induced by ZIKV and DENV.

2022-06-29 | PXD026909 | Pride

Proteomic changes of ZIKV strains and DENV in neural stem cells

2022-06-29 | PXD026825 | Pride

Gene expression analysis of astrocytes differentiated from iPS cells generated from monozygotic twins discordant for schizophrenia

Project description:Previous studies have implicated several brain cell types in schizophrenia, but the genetic impact of astrocytes is unknown. Considering their high complexity in humans, astrocytes are likely key determinants of neurodevelopmental diseases. Human induced pluripotent stem cell (hiPSC) -derived astrocytes differentiated from five monozygotic twin pairs discordant for schizophrenia and five healthy subjects were studied for alterations related to high genetic risk and clinical manifestation of SCZ in astrocyte transcriptomics.

2021-12-20 | GSE191248 | GEO