Dataset Information

Proteomic Analysis of Oil-Roasted Cashew Using a Customized Database

ABSTRACT: Cashew is one of the most prevalent causes of tree nut allergies. However, the cashew proteome is far from complete, which limits the quality of peptide identification in mass spectrometric analyses. In this study, bioinformatics tools were utilized to construct a customized cashew protein database and improve sequence quality for proteins of interest, based on a publicly available cashew genome database. As a result, two additional isoforms for cashew 2S albumins and five other isoforms for cashew 11S proteins were identified, along with several other potential allergens. Using the optimized protein database, the protein profiles of cashew nuts subjected to different oil-roasting conditions (138 °C and 166 °C for 2-10 minutes) were analyzed using discovery LC-MS/MS analysis. The results showed that cashew 2S protein is most heat-stable, followed by 11S and 7S proteins, though protein isoforms might be affected differently. Preliminary target peptide selection indicated that out of the 29 potential targets, 18 peptides were derived from the newly developed database. In the evaluation of thermal processing effects on cashew proteins, several Maillard reaction adducts were also identified. The cashew protein database developed in this study allows for comprehensive analyses of cashew proteome and development of high-quality allergen detection method.

INSTRUMENT(S): Q Exactive HF, Q Exactive

ORGANISM(S): Anacardium Occidentale

SUBMITTER: Shimin Chen

LAB HEAD: Melanie Downs

PROVIDER: PXD027543 | Pride | 2022-06-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20190226_SC_C1_0-75ug.raw	Raw
	20190226_SC_C1_0-75ug_2.raw	Raw
	20190226_SC_C2_0-75ug.raw	Raw
	20190226_SC_C2_0-75ug_2.raw	Raw
	20190427_SC_Cashew_Chy_1A.raw	Raw

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Publications

Proteomic Analysis of Oil-Roasted Cashews Using a Customized Allergen-Focused Protein Database.

Chen Shimin S Downs Melanie L ML

Journal of proteome research 20220603 7

Cashews are one of the most prevalent causes of tree nut allergies. However, the cashew proteome is far from complete, which limits the quality of peptide identification in mass spectrometric analyses. In this study, bioinformatics tools were utilized to construct a customized cashew protein database and improve sequence quality for proteins of interest, based on a publicly available cashew genome database. As a result, two additional isoforms for cashew 2S albumins and five other isoforms for c ...[more]

PMID: 35658452

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