Proteomics profiling based on TMT and PRM reveal crucial biological processes in the leaves of Sophora alopecuroides under salt stress

ABSTRACT: This project was based on Tandem Mass Tag (TMT) and Parallel Reaction Monitoring (PRM) technologies to study the changes in protein expression of leaves in Sophora alopecuroides under 150 mM NaCl condition treated for 3 days and 7 days. It was worthy of attention that the expression of several transporters related to the second messenger signaling pathway, such as the phosphatidylinositol signaling system, cAMP signaling pathway, and calcium signaling pathway had changed when treated for 3 d. However, the expression of some transferase, oxidoreductase, dehydrogenase was involved in the biosynthesis of flavonoids, alkaloids, phenylpropanoids and certain amino acid metabolisms had changed when treated for 7 d. This study analyzed the proteomic profiling of Sophora alopecuroides in response to salt stress using high-throughput omics technology firstly. Many potential genes were discovered that might be involved in salt stress, which laid the foundation for further research on their functions. All the results are of great significance for the researchers who are focus on the molecular genetic machnisms of abiotic stresses in plants, especially under salt stress.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Sophora Alopecuroides

TISSUE(S): Leaf

SUBMITTER: Tian-li Ma  

LAB HEAD: Ping Liu

PROVIDER: PXD027627 | Pride | 2022-02-14

REPOSITORIES: pride