Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PROTEOMIC CHARACTERIZATION OF DRY BLOOD SPOTS OF HEALTHY WOMEN DURING SIMULATION THE MICROGRAVITY EFFECTS USING DRY IMMERSION

ABSTRACT: This study is unique in that it was the first time that it was the female response to microgravity simulated by 3-day dry immersion that was analyzed. The result of the choice of the object of research fell on dry blood spots, since the technique of capillary blood sampling is less invasive and allows sampling with a greater frequency. This allows researchers to analyze the changes caused by dry immersion in stages and observe the unfolding of the adaptive response taking into account the time of exposure to the experimental conditions.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood

SUBMITTER: Alexander Brzhozovskiy

LAB HEAD: Alexey Kononikhin

PROVIDER: PXD027654 | Pride | 2022-02-17

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DBS_immersion_1_1_BB6_1_2772.mgf	Mgf
	DBS_immersion_1_1_BB6_2_2773.mgf	Mgf
	DBS_immersion_1_1_RB1_1_2234.mgf	Mgf
	DBS_immersion_1_1_RB1_2_2235.mgf	Mgf
	DBS_immersion_1_2_RC1_1_2247.mgf	Mgf

Items per page:

1 - 5 of 70

Publications

Proteomic Characterization of Dry Blood Spots of Healthy Women During Simulation the Microgravity Effects Using Dry Immersion.

Kashirina Daria N DN Brzhozovskiy Alexander G AG Sun Wen W Pastushkova Ludmila Kh LK Popova Olga V OV Rusanov Vasiliy B VB Nikolaev Evgeny N EN Larina Irina M IM Kononikhin Alexey S AS

Frontiers in physiology 20220111

PMID: 35087415

Similar Datasets

Proteomic Characterization of Dry Blood Spots of Healthy Women During Simulation the Microgravity Effects Using Dry Immersion.

Project description: Not available

| S-EPMC8787266 | biostudies-literature

Rapid changes in transcriptomic profile and mitochondrial function in human soleus muscle after three-day dry immersion

Project description:We aimed to explore the effect of the 3-day dry immersion - a model of physical unloading - on mitochondrial function, transcriptomic and proteomic profiles in a slow-twitch m. soleus of six healthy females. We registered that a marked reduction (25-34%) in the ADP-stimulated respiration in permeabilized muscle fibers was not accompanied by a decrease in the content of mitochondrial enzymes (mass spectrometry-based quantitative proteomics), hence it is related to the disruption in regulation of respiration. We detected a widespread change in the transcriptomic profile (RNA-seq) upon dry immersion. Down-regulated mRNAs were strongly associated with mitochondrial function, as well as with lipid metabolism, glycolysis, insulin signaling, and various transporters. Despite the substantial transcriptomic response, we found no effect on the content of highly abundant proteins (sarcomeric, mitochondrial, chaperon and extracellular matrix-related, etc.) that may be explained by long half-life of these proteins. We suggest that during short-term disuse the content of some regulatory (and usually low abundant) proteins such as cytokines, receptors, transporters, transcription regulators is largely determined by its mRNA concentration. These mRNAs revealed in our work may serve as putative targets for the future studies aimed at developing the approaches for the prevention of muscle deconditioning-induced by disuse.

2022-11-02 | GSE216845 | GEO

Characterization of Bovine Dry Secretions and their Proteome from the End of Lactation Through Day 21 of the Dry Period

Project description:The dry period in cow are critical to mammary health and the success of the next lactation. The expression of proteins the dry period progresses need to be characterized to provide information on involution and immune functions during the dry period. Total protein and lactoferrin concentrations rose significantly by days 10 and 21 after cessation of lactation. Using the day 21 dry secretions we examined their ability to inhibit bacterial growth. Bacterial growth inhibition was not correlated with lactoferrin for 4 coliforms tested nor for all 7 mastitis pathogens. Label free mass spectroscopy was used to quantify changes in whey proteins from milk/dry secretions collected on days 0, 3,10, and 21 of the dry period. 776 proteins were identified. 109 proteins were upregulated at one or more times in the dry period. The most significate enriched GO functional terms for the up-regulated proteins were immune function and stress related. 68 proteins were down regulated in the dry period. The most significant enriched GO functional terms for the down-regulated proteins were stress and immune function related. 12 proteins were significantly correlated with coliform growth. The dry secretion composition changes we found provide information for understanding the dry period. The sample code for the MS raw files is as follows. The 3 technical replicates are denoted as a letter A, B and C. The number following is the cow identification number for 11 cows used. The final two-digit number after the underscore is the day sampled where _01 = day 1, _03 = day 3, _10 = day 10 and _21 = day 21 of dry period. For example, A1313_01 is technical replicate A for cow 1313 collected on day 1. B1313_03 is technical replicate B for cow 1313 collected on day 3.

2020-05-20 | PXD017837 | Pride

Mapping differences in salivary gland secretions using dried saliva spots

Project description:Dried saliva spot (DSS) sampling is a minimally invasive extraction technique that allows for the spatial mapping of salivary protein distribution in the oral cavity. In conjunction with untargeted nanoLC-Orbitrap-MS/MS analysis, we used DSS to compare the unstimulated proteomes secreted by the parotid and submandibular/sublingual salivary glands

2019-09-04 | PXD012792 | Pride

YDRseries4, Comparison commercial dry active yeast to BY4743

Project description:This series represents the analysis of a commercial dry active yeast (purchased locally). The genetics of this sample is unknown. Keywords = commercial Keywords = dry active yeast Keywords: other

2005-08-01 | GSE1314 | GEO

miR204-containing exosome ameliorates GVHD-associated dry eye disease

Project description:The graft-versus-host disease (GVHD) associated dry eye disease usually leads to refractory pain and visual impairment with limited treatments currently. Here we found exosome derived from mesenchymal stromal cell (MSC-exo) administered as eye drops significantly alleviates GVHD-associated dry eye disease in human and mouse models. To find out the essential elements during exosome treatment, we performed miRNA sequencing of exosomes derived from MSCs and L929 cells, and identified miR-204 in MSC-exo benefited the recovery of dry eye, which targeted IL-6/IL-6R/Stat3 signaling. Blockade of miR-204 abolished the therapeutic effect of MSC-exo while miR-204 overexpression from L929-exo markedly attenuates dry eye. Thus MSC-exo eye drops are efficacious in treating GVHD-associated dry eye and highlight miR-204 as a potential therapeutic agent.

2022-01-19 | GSE166585 | GEO

Transcriptome analysis of Arabidopsis dry seeds

Project description:We report the dry seed transcriptome of 13 wild type accessions of Arabidopsis thaliana

2022-06-06 | GSE179008 | GEO

McMurdo dry valleys

Project description:McMurdo dry valleys | Raw sequence reads

| PRJNA788986 | ENA

McMurdo Dry Valleys

Project description:McMurdo Dry Valleys Raw sequence reads

| PRJNA527658 | ENA

Elephant Head Dry Permafrost

Project description:Elephant Head Dry Permafrost Microbiota