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A proteomic approach for unravelling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica survival and virulence

ABSTRACT: Please provide an overall description of your study, think something similar in scope to the manuscript abstractCFIm25 is essential for recruitment of other polyadenylation factors, poly(A) site selection, pre-mRNA cleavage and poly(A) tail synthesis. We recently reported that EhCFIm25 silencing in Entamoeba histolytica, the protozoan responsible for amebic dysentery, affects trophozoite proliferation and death, and reduces parasite virulence, indicating its relevance as a new biochemical target in this human pathogen. Here, we performed a shotgun proteomic analysis to evidence the impact of EhCFIm25 silencing on protein expression that can explain the observed phenotype

INSTRUMENT(S): Synapt G2 MS

ORGANISM(S): Entamoeba Histolytica Hm-1:imss-a

SUBMITTER: Rodolfo G Avila-Bonilla

LAB HEAD: Laurence A. Marchat

PROVIDER: PXD027784 | Pride | 2021-08-26

REPOSITORIES: Pride

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			Action	DRS
	20200130_138_Control.raw.zip	Raw
	20200130_139_gfp.raw.zip	Raw
	20200130_140_EhCflm25.raw.zip	Raw
	AbsQuant_Trofozoitos_Ehistolytica_EhCflm25_peptides.DerivedFromSearchdfd98be6-d297-400a-9a88-443f190daf7f.mgf	Mgf
	AbsQuant_Trofozoitos_Ehistolytica_EhCflm25_proteins.mzid.gz	Mzid

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