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Stallion sperm proteins in 10 stallions with different motility

ABSTRACT: Using a split sample design 30 ejaculates from 10 different stallions were analyzed as fresh spermatozoa, and another aliquot from the same ejaculate was analyzed as a frozen thawed sample. The proteome was studied under both conditions using UHPLC/MS/MS and bioinformatic analysis conducted to identify discriminant variables between both conditions.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Sperm

SUBMITTER: FERNANDO JUAN PEÑA VEGA

LAB HEAD: FERNANDO JUAN PEÑA VEGA

PROVIDER: PXD025807 | Pride | 2021-07-27

REPOSITORIES: Pride

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			Action	DRS
	SpecFeaturesMS1.tsv	Tabular
	p100.mzXML	Mzxml
	p101.mzXML	Mzxml
	p102.mzXML	Mzxml
	p103.mzXML	Mzxml

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Publications

Proteins involved in mitochondrial metabolic functions and fertilization predominate in stallions with better motility.

Gaitskell-Phillips Gemma G Martín-Cano Francisco E FE Ortiz-Rodríguez José M JM Silva-Rodríguez Antonio A da Silva-Álvarez Eva E Rojo-Domínguez Patricia P Tapia José A JA Gil Maria C MC Ortega-Ferrusola Cristina C Peña Fernando J FJ

Journal of proteomics 20210721

Even in stallions with sperm quality within normal reference ranges at ejaculation, subtle differences in sperm quality exist that in many cases lead to reduced time frames for conservation of the ejaculate and/or reduced fertility. The spermatozoon is a cell highly suitable for proteomics studies, and the use of this technique is allowing rapid advances in the understanding of sperm biology. The aim of the present study was to investigate differences among stallions of variable sperm quality (b ...[more]

PMID: 34298182

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