Dataset Information

Essential role of Cp190 in physical and regulatory boundary formation in Drosophila

ABSTRACT: Animal genomes fold into contact domains defined by enhanced internal contact frequencies with debated functions in establishing independent gene regulatory domains. A large fraction of contact domains in mammals are formed by stalling of chromosomal loop-extruding cohesin by CTCF at domain boundaries. 90% of domain boundaries in Drosophila form CTCF-independently, and other proteins were proposed to form chromosomal loops with dual functions of segregating promoters from inappropriate regulatory elements and connecting distal regulatory elements to their correct targets. Here, we genetically ablate the ubiquitous boundary-associated factor Cp190 and assess impacts on genome folding and transcriptional regulation in embryos. Our results reveal that Cp190 is a major factor required for contact domain boundary formation and gene insulation in Drosophila.

INSTRUMENT(S): timsTOF Pro, LTQ Orbitrap Velos

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo

SUBMITTER: Patrice Waridel

LAB HEAD: Maria Cristina Gambetta

PROVIDER: PXD027795 | Pride | 2022-05-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	10610-11060.zip	Other
	1128_4-22-2021_Wang_13236-mIST_140min_RB1.d.zip	Other
	1131_4-22-2021_Wang_13237-mIST_140min_RB2.d.zip	Other
	1134_4-22-2021_Wang_13238-mIST_140min_RB3.d.zip	Other
	1268_5-25-2021_Wang_13305_140min_2ul_RB1.d.zip	Other

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Publications

Essential role of Cp190 in physical and regulatory boundary formation.

Kaushal Anjali A Dorier Julien J Wang Bihan B Mohana Giriram G Taschner Michael M Cousin Pascal P Waridel Patrice P Iseli Christian C Semenova Anastasiia A Restrepo Simon S Guex Nicolas N Aiden Erez Lieberman EL Gambetta Maria Cristina MC

Science advances 20220513 19

Boundaries in animal genomes delimit contact domains with enhanced internal contact frequencies and have debated functions in limiting regulatory cross-talk between domains and guiding enhancers to target promoters. Most mammalian boundaries form by stalling of chromosomal loop-extruding cohesin by CTCF, but most <i>Drosophila</i> boundaries form CTCF independently. However, how CTCF-independent boundaries form and function remains largely unexplored. Here, we assess genome folding and developme ...[more]

PMID: 35559678

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