Dataset Information

Proteomic study of apheresis platelets made HLA class I deficient for transfusion of refractory patients

ABSTRACT: Refractoriness can occur after repeated PLT transfusions because of alloimmunization to HLA class I antigens on transfused PLTs and generation of anti-HLA antibodies that bind to the foreign PLTs and initiate their destruction. Such refractoriness can be overcome by provision of HLA-matched PLTs from HLA typed donors. However, since the procedure is both expensive and time-consuming, an alternative approach is to deplete PLTs of HLA class I molecules by a brief treatment with citric acid, on ice at low pH. This is shown to be feasible without damaging PLT function

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Platelet, Blood

SUBMITTER: Sachin Singh

LAB HEAD: Geir Hetland

PROVIDER: PXD027893 | Pride | 2022-02-14

REPOSITORIES: Pride

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Proteomic study of apheresis platelets made HLA class I deficient for transfusion of refractory patients.

Mirlashari Mohammad Reza MR Vetlesen Annette A Nissen-Meyer Lise Sofie H LSH Stensland Maria Ekman ME Singh Sachin Kumar SK Nyman Tuula Anneli TA Hetland Geir G

Proteomics. Clinical applications 20210922 6

<h4>Purpose</h4>Refractoriness can occur after repeated platelet (PLT) transfusions because of alloimmunization to HLA class I antigens on transfused PLTs and generation of anti-HLA antibodies that bind to the foreign PLTs and initiate their destruction. Such refractoriness can be overcome by provision of HLA-matched PLTs from HLA typed donors. However, since the procedure is both expensive and time-consuming, an alternative approach is to deplete PLTs of HLA class I molecules by a brief treatme ...[more]

PMID: 34510746

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