Dataset Information

Systemic effects of hemorrhagic snake venom metalloproteinases: untargeted peptidomics to explore the pathodegradome of plasma proteins

ABSTRACT: In this study we investigated the proteolysis of human plasma proteins by HF3, an hemorrhagic metalloproteinase from B. jararaca venom. For this purpose, approaches for the depletion of the most abundant proteins and for the enrichment of low abundant proteins of human plasma were used to minimize the dynamic range of protein concentration, in order to assess the proteolytic activity of HF3 on a wide spectrum of proteins, and to detect the degradation products by mass spectrometry by untargeted peptidomics.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Disease Free

SUBMITTER: Alison Chaves

LAB HEAD: Solange Maria de Toledo Serrano

PROVIDER: PXD027997 | Pride | 2022-02-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	P_20-MAP-D__CONTROL_01.dat	Other
	P_20-MAP-D__CONTROL_01.raw	Raw
	P_20-MAP-D__CONTROL_02.dat	Other
	P_20-MAP-D__CONTROL_02.raw	Raw
	P_20-MAP-D__CONTROL_03.dat	Other

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Publications

Systemic Effects of Hemorrhagic Snake Venom Metalloproteinases: Untargeted Peptidomics to Explore the Pathodegradome of Plasma Proteins.

Bertholim Luciana L Chaves Alison F A AFA Oliveira Ana K AK Menezes Milene C MC Asega Amanda F AF Tashima Alexandre K AK Zelanis Andre A Serrano Solange M T SMT

Toxins 20211028 11

Hemorrhage induced by snake venom metalloproteinases (SVMPs) is a complex phenomenon that involves capillary disruption and blood extravasation. HF3 (hemorrhagic factor 3) is an extremely hemorrhagic SVMP of <i>Bothrops jararaca</i> venom. Studies using proteomic approaches revealed targets of HF3 among intracellular and extracellular proteins. However, the role of the cleavage of plasma proteins in the context of the hemorrhage remains not fully understood. The main goal of this study was to an ...[more]

PMID: 34822548

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