Dataset Information

Comparative Proteomic Analysis to Reveal Key Maize Low Nitrogen Responsive Genes

ABSTRACT: Two maize hybrid cultivars contrasting in low nitrogen tolerance (low nitrogen-tolerant XY335 and low nitrogen-sensitive HN178) were used in this study . The experiment was carried out at Xinji Experimental Station (43º31′N, 124º48′E) of Hebei Agricultural University. The top 0-20 cm of the soil used contained organic matter 17.79 g·kg-1, total nitrogen 1.21 g·kg-1, alkali hydrolyzed nitrogen 64.9 mg.kg-1, available phosphorus 23.8 mg·kg-1, and available potassium 120.6 mg·kg-1. The experiment adopted a split plot design, with varieties as the main plot and nitrogen fertilizer as the sub-plot. There were 2 varieties for testing: XY335 and HN138. Two levels of nitrogen supply: N0 (0 kg N ha-1) and N240 (240 kg N ha-1), replicated three times. Each plot had 6 rows, with the row length measuring 20 m, and the row spacing of 60 cm, giving the plot area of 72 m2. The planting density was 67,500 plants ha-1. Nitrogen fertilizer used was urea (46% N), and 50% was applied before sowing and at the flared stage, respectively. During the grain filling stage, leaf tissues of three biological replicates were collected from control and treatment conditions, and immediately frozen in liquid nitrogen for subsequent proteomics analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Plant Cell, Leaf

DISEASE(S): Disease Free

SUBMITTER: Jiao Li

LAB HEAD: Huijun Duan

PROVIDER: PXD028027 | Pride | 2022-05-20

REPOSITORIES: Pride

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			Action	DRS
	20191018_TMT_10_120min_Ci06LST_bjhb1_1.raw	Raw
	20191018_TMT_10_120min_Ci06LST_bjhb1_10.raw	Raw
	20191018_TMT_10_120min_Ci06LST_bjhb1_2.raw	Raw
	20191018_TMT_10_120min_Ci06LST_bjhb1_3.raw	Raw
	20191018_TMT_10_120min_Ci06LST_bjhb1_4.raw	Raw

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Publications

Comparative Proteomic Analysis of Two Contrasting Maize Hybrids' Responses to Low Nitrogen Stress at the Twelve Leaf Stage and Function Verification of <i>ZmTGA</i> Gene.

Wang Yafei Y Wang Nan N Liu Songtao S Dong Anyi A Zenda Tinashe T Liu Xinyue X Li Jiao J Duan Huijun H

Genes 20220411 4

Nitrogen is one of the essential nutrients for plant growth and development. However, large amounts of nitrogen fertilizer not only increase the production costs, but also lead to serious environmental problems. Therefore, it is particularly important to reduce the application of nitrogen fertilizer and develop maize varieties with low nitrogen tolerance. The aim of this study was to determine the phenotypic and proteomic alterations of maize affected by nitrogen deficiency and to elucidate the ...[more]

PMID: 35456476

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