Proteomics

Dataset Information

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Wild-type and Pgrmc1 KO mouse liver membrane LC-MSMS with isobaric tagging

ABSTRACT: Progesterone receptor membrane component 1 (PGRMC1) is a heme binding protein implicated in a wide range of cellular functions. Our previous studies showed that PGRMC1 binds to cytochromes P450 in yeast and mammalian cells and promotes activity of these enzymes. Recently, the paralog PGRMC2 was shown to function as an intracellular heme chaperone. Here, we examined the function of Pgrmc1 in mouse liver by measuring levels of membrane proteins in livers from wild-type and Pgrmc1 knockout mice. These experiments revealed that Pgrmc1 knockout mouse livers contained reduced levels of cytochrome P450 enzymes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Peter Espenshade  

LAB HEAD: Peter J. Espenshade

PROVIDER: PXD028238 | Pride | 2022-02-16

REPOSITORIES: Pride

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Dataset's files

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Action DRS
160719_EspenshadeP_MM_TMT_Labels_REP2.xlsx Xlsx
170223_EspenshadeP_MM_TMT_10plex_labeling_KEY_REP1.csv Csv
LD-CS-160720_EspenshadeP_MM_Prerun_BSA_REP2.raw Raw
LD-CS-160720_EspenshadeP_MM_Prerun_Blank_REP2.raw Raw
LD-CS-160720_EspenshadeP_MM_TMT_15pBRP_REP2.raw Raw
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Publications

Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism.

McGuire Meredith R MR   Mukhopadhyay Debaditya D   Myers Stephanie L SL   Mosher Eric P EP   Brookheart Rita T RT   Kammers Kai K   Sehgal Alfica A   Selen Ebru S ES   Wolfgang Michael J MJ   Bumpus Namandjé N NN   Espenshade Peter J PJ  

The Journal of biological chemistry 20211020 5

Progesterone receptor membrane component 1 (PGRMC1) is a heme-binding protein implicated in a wide range of cellular functions. We previously showed that PGRMC1 binds to cytochromes P450 in yeast and mammalian cells and supports their activity. Recently, the paralog PGRMC2 was shown to function as a heme chaperone. The extent of PGRMC1 function in cytochrome P450 biology and whether PGRMC1 is also a heme chaperone are unknown. Here, we examined the function of Pgrmc1 in mouse liver using a knock  ...[more]

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