Proteomics

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Late-stage modification of peptides and proteins at cysteine with diaryliodonium salts

ABSTRACT: The modification of peptides and proteins has emerged as a powerful means to efficiently prepare high value bioconjugates for a range of applications in chemical biology and for the development of next-generation therapeutics. Herein, we report a novel method for the chemoselective late-stage modification of peptides and proteins at cysteine in aqueous buffer with suitably functionalised diaryliodonium salts, furnishing stable thioether-linked synthetic conjugates. The power of this new platform is showcased through the late-stage modification of the affibody zEGFR and the histone protein H2A.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mark Larance  

LAB HEAD: Mark Larance

PROVIDER: PXD028382 | Pride | 2022-02-16

REPOSITORIES: Pride

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20210826_Eclipse_ML_DF_EXP2_H2A-Ketone.raw Raw
20210826_Eclipse_ML_DF_EXP2_H2A-Pen.raw Raw
20210826_Eclipse_ML_DF_EXP2_SB-P29.raw Raw
20210826_Eclipse_ML_DF_EXP2_SB-P30.raw Raw
20210826_Eclipse_ML_DF_EXP2_SB-P31.raw Raw
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Late-stage modification of peptides and proteins at cysteine with diaryliodonium salts.

Byrne Stephen A SA   Bedding Max J MJ   Corcilius Leo L   Ford Daniel J DJ   Zhong Yichen Y   Franck Charlotte C   Larance Mark M   Mackay Joel P JP   Payne Richard J RJ  

Chemical science 20210930 42

The modification of peptides and proteins has emerged as a powerful means to efficiently prepare high value bioconjugates for a range of applications in chemical biology and for the development of next-generation therapeutics. Herein, we report a novel method for the chemoselective late-stage modification of peptides and proteins at cysteine in aqueous buffer with suitably functionalised diaryliodonium salts, furnishing stable thioether-linked synthetic conjugates. The power of this new platform  ...[more]

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