Dataset Information

Proteomic characterization of artificially aged seed in Nelumbo Nucifera

ABSTRACT: The long-lived sacred lotus (Nelumbo nucifera) fruit provides a good platform for uncovering aging-related mechanism in extension of seed-life and improving of food quality of economic crops during storage. Here, we investigated the proteome of artificial aging lotus seed to uncover the aging -resistance mechanism. Proteome was performed on the seeds embryo axis treated at 80C for 0, 12 and 24 Hours. We identified 4667 proteins, and found 383 and 37 proteins (folds>1.3, FDR<0.01) were differentially expressed from 0-12h and 12-24h treatment, respectively. This proteome data will provide a better understanding and give further insights on lotus seed aging-resistance.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Nelumbo Nucifera (sacred Lotus)

TISSUE(S): Plant Cell, Embryo

SUBMITTER: Dongli He

LAB HEAD: Pingfang Yang

PROVIDER: PXD028436 | Pride | 2024-12-23

REPOSITORIES: Pride

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TMT-based quantitative proteomic and physiological analyses on lotus plumule of artificially aged seed in long-living sacred lotus Nelumbo nucifera.

He Dongli D Cai Mengmeng M Liu Meihui M Yang Pingfang P

Journal of proteomics 20220927

Seed longevity is important for the maintenance of seed nutritional quality, vigor, and germination potential during storage. Sacred lotus is known as one of the longest living seeds in the world and their ability to maintain longevity has been widely investigated. In this study, a suitable controlled deterioration treatment (CDT) method was first established to evaluate the vigor loss of lotus plumule (LP), and then the Tandem Mass Tags (TMT)-based proteomic analysis was performed on LP from th ...[more]

PMID: 36174953

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Project description:Lotus is an aquatic plant that is sensitive to water loss, but its seeds are longevous after seed embryo dehydration and maturation. The great difference between the responses of vegetative organs and seeds to dehydration is related to the special protective mechanism in embryos. In this study, tandem mass tags (TMT)-labeled proteomics and parallel reaction monitoring (PRM) technologies were used to obtain novel insights into the physiological regulatory networks during lotus seed dehydration process. Totally, 60,266 secondary spectra and 32,093 unique peptides were detected. A total of 5,477 reliable proteins and 815 differentially expressed proteins (DEPs) were identified based on TMT data; of these, 582 DEPs were continuously down-regulated and 228 proteins were significantly up-regulated during the whole dehydration process. Bioinformatics and protein-protein interaction network analyses indicated that carbohydrate metabolism (including glycolysis/gluconeogenesis, galactose, starch and sucrose metabolism, pentose phosphate pathway, and cell wall organization), protein processing in ER, DNA repair, and antioxidative events had positive responses to lotus embryo dehydration. On the contrary, energy metabolism (metabolic pathway, photosynthesis, pyruvate metabolism, fatty acid biosynthesis) and secondary metabolism (terpenoid backbone, steroid, flavonoid biosynthesis) gradually become static status during lotus embryo water loss and maturation. Furthermore, non-enzymatic antioxidants and pentose phosphate pathway play major roles in antioxidant protection during dehydration process in lotus embryo; ABA signaling and the accumulation of oligosaccharides, late embryogenesis abundant proteins, and heat shock proteins may be the key factors to ensure the continuous dehydration and storage tolerance of lotus seed embryo. Stress physiology detection showed that H2O2 was the main ROS component inducing oxidative stress damage, and glutathione and vitamin E acted as the major antioxidant to maintain the REDOX balance of lotus embryo during the dehydration process. These results provide new insights to reveal the physiological regulatory networks of the protective mechanism of embryo dehydration in lotus.

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Dataset Information

Proteomic characterization of artificially aged seed in Nelumbo Nucifera

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Publications

TMT-based quantitative proteomic and physiological analyses on lotus plumule of artificially aged seed in long-living sacred lotus Nelumbo nucifera.

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