Dataset Information

Phosphorylation Mapping of Atg13 in Response to Atg1 Activity and Rapamycin Treatment in Budding Yeast

ABSTRACT: We performed phosphorylation site mapping on of budding yeast Atg13 applying a quantitative mass spectrometry-based proteomics approach based on stable isotope labeling with amino acids in cell culture (SILAC). We determined changes in the Atg13 phosphorylation pattern in response of inhibition of kinase Atg1 and starvation conditions (using rapamycin), respectively. To do so, Atg13 was tandem purified via a histidine-biotin (HB) tag from cells growing at the respective experimental condition. Different stimulus-dependent PTM profiles of Atg13 have been identified.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Wolfgang Reiter

LAB HEAD: Claudine Kraft

PROVIDER: PXD028461 | Pride | 2024-01-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	120117V_WR_SILAC_13C_strainWR788__Atg13_-_12C_strainWR792__Atg13_delta_Atg1__both_45min_rapa_TiO2.msf	Msf
	120117V_WR_SILAC_13C_strainWR788__Atg13_-_12C_strainWR792__Atg13_delta_Atg1__both_45min_rapa_TiO2.raw	Raw
	120117V_WR_SILAC_strainWR788__Atg13_-_13C_untreated_-_12C_45min_rapa__TiO2.msf	Msf
	120117V_WR_SILAC_strainWR788__Atg13_-_13C_untreated_-_12C_45min_rapa__TiO2.raw	Raw
	120529V_WR_SILAC_Atg13_strain_WR788__Atg13-HTB_wt__13C__untreated__12C__45min_rapa__10__WH.msf	Msf

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Publications

Decoding the function of Atg13 phosphorylation reveals a role of Atg11 in bulk autophagy initiation.

Bhattacharya Anuradha A Torggler Raffaela R Reiter Wolfgang W Romanov Natalie N Licheva Mariya M Ciftci Akif A Mari Muriel M Kolb Lena L Kaiser Dominik D Reggiori Fulvio F Ammerer Gustav G Hollenstein David M DM Kraft Claudine C

EMBO reports 20240117 2

Autophagy is initiated by the assembly of multiple autophagy-related proteins that form the phagophore assembly site where autophagosomes are formed. Atg13 is essential early in this process, and a hub of extensive phosphorylation. How these multiple phosphorylations contribute to autophagy initiation, however, is not well understood. Here we comprehensively analyze the role of phosphorylation events on Atg13 during nutrient-rich conditions and nitrogen starvation. We identify and functionally c ...[more]

PMID: 38233718

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