Proteomics

Dataset Information

0

Proteome of human branching and non-branching cholangiocyte organoids

ABSTRACT: Current model systems for studying intrahepatic biliary disease fail to recapitulate the architecture of intrahepatic bile ducts. Organoids from intrahepatic cholangiocytes can with a new culture method grow with a branching pattern resembling the structure of intrahepatic bile ducts. This dataset contains data from samples of these organoids and from control organoids cultured without this technique. Culture have been performed using isotope labeled amino acids to help distinguish cell derived proteins from the basement membrane extract used as a culture substrate for the organoids.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Intrahepatic Cholangiocyte

SUBMITTER: Oskar Rosmark  

LAB HEAD: Prof. Luc van der Laan

PROVIDER: PXD028532 | Pride | 2022-05-09

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
1284_01.raw Raw
1284_DKK1.raw Raw
1357_01.raw Raw
1357_DKK1.raw Raw
1359_01.raw Raw
Items per page:
1 - 5 of 14
altmetric image

Publications

Human cholangiocyte organoids show great promise for regenerative therapies and in vitro modeling of bile duct development and diseases. However, the cystic organoids lack the branching morphology of intrahepatic bile ducts (IHBDs). Here, we report establishing human branching cholangiocyte organoid (BRCO) cultures. BRCOs self-organize into complex tubular structures resembling the IHBD architecture. Single-cell transcriptomics and functional analysis showed high similarity to primary cholangioc  ...[more]

Similar Datasets

Transcriptomics Human branching cholangiocyte organoids recapitulate embryogenic bile duct development and provide an unique model to study biliary diseases. RNA-Seq
2022-05-04 | GSE179599 | GEO
Transcriptomics Human branching cholangiocyte organoids recapitulate embryogenic bile duct development and provide an unique model to study biliary diseases. scRNA-Seq
2022-05-04 | GSE179597 | GEO
Transcriptomics Comparison of extrahepatic cholangiocyte organoids and freshly isolated common bile duct cholangiocytes
2017-07-04 | E-MTAB-4591 | biostudies-arrayexpress
Transcriptomics Transcription profiling of undifferentiated human extrahepatic cholangiocyte organoids (ECOs)
2021-01-01 | E-MTAB-9807 | biostudies-arrayexpress
Transcriptomics De novo formation of a functional biliary system by TGFβ-mediated hepatocyte transdifferentiation
2018-05-14 | GSE108315 | GEO
Genomics Distinct Phenotypes of Human Intrahepatic and Extrahepatic Bile duct Organoids and their Applications for Biliary Disease Modeling
| EGAS00001003792 | EGA
Transcriptomics RNA-seq analysis of hilar and peripheral biliary organoids from a mouse model of Alagille syndrome
2023-12-02 | GSE229527 | GEO
Transcriptomics Single-cell RNA sequencing of the human biliary tree
2021-03-12 | E-MTAB-8495 | biostudies-arrayexpress
Transcriptomics Differential gene expression profiling of cultured neu-transformed versus spontaneously-transformed rat cholangiocytes and of corresponding cholangiocarcinomas
2012-06-20 | E-GEOD-38834 | biostudies-arrayexpress
Transcriptomics Transcription profiling of undifferentiated human liver-derived intrahepatic cholangiocyte organoids (ICOs)
2020-07-31 | E-MTAB-9044 | biostudies-arrayexpress