Dataset Information

Toxoplasma gondii MOB1 interactome

ABSTRACT: MOB1 is a conserved protein that regulates cellular proliferation versus apoptosis, centrosome duplication and cellular differentiation in multicellular eukaryotes and also cytokinesis and division axis orientation in unicellular and multicellular eukaryotes. Toxoplasma gondii, an obligate intracellular parasite of veterinary and medical importance, presents one MOB1 protein. T. gondii interconverts between several cellular stages during its life cycle, namely between fast replicating tachyzoite and slow replicating bradyzoite stages during its asexual cycle, a key ability for its success as a parasite. Bradyzoites produce tissue cysts, establishing a chronic infection that enables recrudescence. Conversion is dependent on cell cycle regulation and involves cell differentiation and regulation of replication. This led us to select MOB1 as a strong candidate to be involved in the Toxoplasma replication process. To elucidate how MOB1 acts in T. gondii, we employed a proximity biotinylation method and identified the MOB1 interactome. Toxoplasma gondii RH tachyzoites were transfected with BirA containing plasmid vectors for random integration and two strains were isolated. The control strain expresses a FLAG-BirA recombinant protein while the test strain expresses a FLAG-BirA-MOB1 recombinant protein. Biotinylated proteins were purified using streptavidin-agarose beads. The purified proteins were trypsinized and analyzed by nanoLC-MS/MS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Toxoplasma Gondii

SUBMITTER: Inês Delgado

LAB HEAD: Alexandre Leitão

PROVIDER: PXD028781 | Pride | 2022-02-16

REPOSITORIES: Pride

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Publications

Characterization of a MOB1 Homolog in the Apicomplexan Parasite <i>Toxoplasma gondii</i>.

Delgado Inês L S ILS Tavares Alexandra A Francisco Samuel S Santos Dulce D Coelho João J Basto Afonso P AP Zúquete Sara S Müller Joachim J Hemphill Andrew A Meissner Markus M Soares Helena H Leitão Alexandre A Nolasco Sofia S

Biology 20211126 12

Monopolar spindle One Binder1 (MOB1) proteins are conserved components of the tumor-suppressing Hippo pathway, regulating cellular processes such as cytokinesis. Apicomplexan parasites present a life cycle that relies on the parasites' ability to differentiate between stages and regulate their proliferation; thus, Hippo signaling pathways could play an important role in the regulation of the apicomplexan life cycle. Here, we report the identification of one MOB1 protein in the apicomplexan <i>To ...[more]

PMID: 34943148

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			Action	DRS
	A1_Tg.mzML	Mzml
	A1_Tg.raw	Raw
	A2_Tg.mzML	Mzml
	A2_Tg.raw	Raw
	A3_Tg.mzML	Mzml