Dataset Information

Pandanus amaryllifolius drought stress and recovery LC-MSMS TMT-Labelled

ABSTRACT: The aim of this project is to determine the protein changes of P. amaryllifolius in response to drought stress and during recovery. The protein changes between drought-stressed, well-watered, and recovered plants were evaluated using tandem mass tags (TMT)-based quantitative proteomics. The proteins were extracted from sample by using TCA/Acetone method and digested with trypsin. The digested proteins were then labelled with 10-plex TMT labelling prior fractionation with 12.5%, 17.5%, and 50% ACN with 0.1% triethylamine. The prepared proteins were then subjected to 1D data dependent acquisition (DDA) with nano LC column ionization source was set as positive ion mode, whereby 350-1850 m/z peptide precursors were scanned at 70,000 resolutions. The raw data generated was then searched against Viridiplantae protein sequences downloaded from UniProt. Of the 1,415 differentially abundant proteins, 74 were significantly altered. The majority of proteins differing between them were related to carbon metabolism, photosynthesis, stress response, and antioxidant activity. This is the first study that reports the protein changes in response to drought stress in Pandanus. The data generated provide an insight into the drought-responsive mechanisms in P. amaryllifolius.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Pandanus Amaryllifolius

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Boon Chin Tan

LAB HEAD: Boon Chin Tan

PROVIDER: PXD028784 | Pride | 2022-02-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Pandanus_DroughtRecovery_12p5.mzML	Mzml
	Pandanus_DroughtRecovery_12p5.raw	Raw
	Pandanus_DroughtRecovery_17p5.mzML	Mzml
	Pandanus_DroughtRecovery_17p5.raw	Raw
	Pandanus_DroughtRecovery_50.mzML	Mzml

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Publications

Drought Stress Induces Morpho-Physiological and Proteome Changes of <i>Pandanus amaryllifolius</i>.

Amnan Muhammad Asyraf Mohd MAM Aizat Wan Mohd WM Khaidizar Fiqri Dizar FD Tan Boon Chin BC

Plants (Basel, Switzerland) 20220115 2

Drought is one of the significant threats to the agricultural sector. However, there is limited knowledge on plant response to drought stress and post-drought recovery. <i>Pandanus amaryllifolius</i>, a moderate drought-tolerant plant, is well-known for its ability to survive in low-level soil moisture conditions. Understanding the molecular regulation of drought stress signaling in this plant could help guide the rational design of crop plants to counter this environmental challenge. This study ...[more]

PMID: 35050109

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