Proteomics

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Proteomic and phosphoproteomic profilings in fibroblasts of 11 common mammalian species

ABSTRACT: A central goal of modern biology is to understand the molecular basis of phenotype evolution between species. Previously, the evolutionary profiling at the molecular level has been largely limited to the nucleotide level. Nevertheless, the genotype is translated to proteotype through comprehensive and gene-specific post-transcriptional regulations. Harnessing recently emerged consistent proteomic quantification methods, we here investigate the co-evolution processes across the transcriptomic, proteomic, and phosphoproteomic layers in skin fibroblasts from 11 common mammalian species. Altogether, we have generated a multi-species, multi-omics resource, the analysis of which significantly facilitates the understanding of gene expression, proteotype co-evolution, and biodiversity in mammals.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Rattus Norvegicus (rat) Canis Lupus Equus Caballus (horse) Homo Sapiens (human) Bos Taurus (bovine) Felis Catus (cat) (felis Silvestris Catus) Ovis Aries Oryctolagus Cuniculus (rabbit) Sus Scrofa (pig) Macaca Mulatta (rhesus Macaque) Monodelphis Domestica

TISSUE(S): Cell Culture

SUBMITTER: Wenxue Li  

LAB HEAD: Yansheng Liu

PROVIDER: PXD028979 | Pride | 2022-09-22

REPOSITORIES: Pride

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Dataset's files

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Action DRS
20200218_CatBSF_1.raw Raw
20200218_CatBSF_2.raw Raw
20200218_CatBSF_3.raw Raw
20200218_CowBSF_A.raw Raw
20200218_CowBSF_B.raw Raw
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Publications

Proteotype coevolution and quantitative diversity across 11 mammalian species.

Ba Qian Q   Hei Yuanyuan Y   Dighe Anasuya A   Li Wenxue W   Maziarz Jamie J   Pak Irene I   Wang Shisheng S   Wagner Günter P GP   Liu Yansheng Y  

Science advances 20220909 36

Evolutionary profiling has been largely limited to the nucleotide level. Using consistent proteomic methods, we quantified proteomic and phosphoproteomic layers in fibroblasts from 11 common mammalian species, with transcriptomes as reference. Covariation analysis indicates that transcript and protein expression levels and variabilities across mammals remarkably follow functional role, with extracellular matrix-associated expression being the most variable, demonstrating strong transcriptome-pro  ...[more]

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