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Single-cell RNA-seq of of dermal fibroblasts (second replicate)

ABSTRACT: Dermal fibroblasts from human, rhesus macaque, mouse and rat, stimulated with dsRNA (poly I:C) in a time course of 0,4 and 8 hours, profiled using the Smart-seq2 protocol.
The innate immune response - the expression programme that is initiated once a pathogen is sensed - is known to be variable among responding cells, as well as to rapidly evolve in the course of mammal evolution. To study the transcriptional divergence and cell-to-cell variability of this response, we stimulated dermal fibroblast cells from two primates (human and macaque) and two rodents (mouse and rat) with dsRNA - a mimic of viral RNA that elicits a rapid innate immune response. Subsequently, we profiled the response using bulk RNA-seq, scRNA-seq and ChIP-seq across the four species and across different time points.

INSTRUMENT(S): Illumina HiSeq 2500, Beckman Coulter MoFlo XDP

ORGANISM(S): Mus musculus

SUBMITTER: Tzachi Hagai

PROVIDER: E-MTAB-6066 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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single-cell RNA-seq of of dermal fibroblasts

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