Proteomics

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New insights on the role of HCN in root hair elongation through single cell proteomics.


ABSTRACT: In order to identify the possible protein targets of cyanide in the regulation of root hair, we have carried out a single cell proteomic approach to characterize the protein atlas in wild type root hair cells compared to the cas-c1 mutant cells. Root hair specific cells were obtained from isolated protoplast from root tissues of Arabidopsis wt-pCOBL9:GFP and cas-c1-pCOBL9:GFP lines. To analyze the effect of the CAS-C1 mutation, we isolated 1 x 106 root hair cells by Fluorescence-activated cell sorting (FACS) from three independent replicate of wt-pCOBL9:GFP and cas-c1-pCOBL9:GFP roots. The extracted proteins from each sample were trypsin-digested and the digested peptides were analyzed by liquid chromatography-high resolution mass spectrometry (LC-MS/MS) for protein identification. In wild type samples, we have identified 3829 unique proteins at a false discovery rate below 1% (FDR<1%), that represent almost 10% of the total Arabidopsis proteome. In protein extract of the cas-c1-pCOBL9:GFP roots samples, we have identified 3972 proteins of which 3515 were commons in both cell types, 310 were only identified y wild type and 457 only in cas-c1 mutant (Fig. 5).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Root

SUBMITTER: Luis C. Romero  

LAB HEAD: Luis C. Romero

PROVIDER: PXD028991 | Pride | 2024-01-26

REPOSITORIES: Pride

Dataset's files

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Action DRS
CAS123_04.group Other
CAS1_04.group Other
CAS2_04.group Other
ID39352_02_06069L_5064_072518.raw Raw
ID39352_02_06069L_5064_072518_1.mgf Mgf
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Publications

New Insights on the Role of ß-Cyanoalanine Synthase CAS-C1 in Root Hair Elongation through Single-Cell Proteomics.

Arenas-Alfonseca Lucía L   Yamada Masashi M   Romero Luis C LC   García Irene I  

Plants (Basel, Switzerland) 20231202 23


(1) Background: Root hairs are specialized structures involved in water and plant nutrient uptake. They elongate from epidermal cells following a complex developmental program. ß-cyanoalanine synthase (CAS), which is mainly involved in hydrogen cyanide (HCN) detoxification in <i>Arabidopsis thaliana</i>, plays a role in root hair elongation, as evidenced by the fact that <i>cas-c1</i> mutants show a severe defect in root hair shape. In addition to root hairs, CAS C1 is expressed in the quiescent  ...[more]

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