Proteomics

Dataset Information

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Response of Haloferax volcanii to low-iron stress


ABSTRACT: We analyzed the response of the haloarchaeal model organism Haloferax volcanii to low iron stress on the proteome level by label-free mass spectrometry. Cells grown in minimal medium with normal iron levels were compared to those grown under low iron conditions. We separated the samples into soluble and membrane fraction to focus specifically on import/export processes which are frequently associated with metal uptake. Using a comprehensive annotated spectral library and data-independent acquisition mass spectrometry, we identified significant changes to both the membrane and the soluble proteomes of Haloferax volcanii under iron starvation.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Haloferax Volcanii (halobacterium Volcanii)

SUBMITTER: Christof Lenz  

LAB HEAD: Christof Lenz

PROVIDER: PXD029036 | Pride | 2024-08-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20200508_FP_HALVO_cRAP_iRT.fasta Fasta
20200512_161018_Jevtic_Iron_and_Rich_Medium.sne Other
20200513_153733_Jevtic_Results_Summary_3.xlsx Xlsx
Runs_and_Identifications.xlsx Xlsx
Z_Jevtic_071215_HALVO_F01_01.wiff Wiff
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