Response of Haloferax volcanii to low-iron stress
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ABSTRACT: We analyzed the response of the haloarchaeal model organism Haloferax volcanii to low iron stress on the proteome level by label-free mass spectrometry. Cells grown in minimal medium with normal iron levels were compared to those grown under low iron conditions. We separated the samples into soluble and membrane fraction to focus specifically on import/export processes which are frequently associated with metal uptake. Using a comprehensive annotated spectral library and data-independent acquisition mass spectrometry, we identified significant changes to both the membrane and the soluble proteomes of Haloferax volcanii under iron starvation.
INSTRUMENT(S): TripleTOF 5600
ORGANISM(S): Haloferax Volcanii (halobacterium Volcanii)
SUBMITTER: Christof Lenz
LAB HEAD: Christof Lenz
PROVIDER: PXD029036 | Pride | 2024-08-04
REPOSITORIES: Pride
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