Multi-omic analysis reveals the biochemical changes underpinning the varied phenotypes of the Arabidopsis long-period mutant rve 4,6,8

ABSTRACT: Plants are able to sense changes in their light environments, such as the onset of day and night, as well as anticipate these changes in order to adapt and survive. Central to this ability is the plant circadian clock, a molecular circuit that precisely orchestrates plant cell processes over the course of a day. REVEILLE proteins (RVEs) are recently discovered members of the plant circadian circuitry that activate the evening complex and PRR genes to maintain regular circadian oscillation. The RVE4, 6 and 8 proteins in particular have been shown to limit the length of the circadian period, with rve4/6/8 plants possessing an elongated period as well as increased leaf surface area and biomass relative to wild-type Col-0 plants. Here, using a multi-omics approach consisting of phenomics, transcriptomics, proteomics, and metabolomics we demonstrate how RVE8-like proteins impact diel plant cell function and draw novel connections to a number of plant cell processes that underpin the growth and development phenotypes observed in rve4/6/8 plants. In particular, we reveal that loss of RVE8-like proteins results in altered carbohydrate and lipid metabolism, including a starch excess phenotype at ZT0. We further demonstrate that RVE8-like proteins has an unique impact on the abundance and phosphorylation 26S proteasome subunits, in addition to impacting the abundance and/or phosphorylation status of a number of protein kinases. . Overall, this robust, multi-omic dataset provides substantial new molecular insights into RVE8-like protein function demonstrating the far reaching impact RVE8-like proteins have on the diel plant cell environment.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Rosette

SUBMITTER: Richard Uhrig  

LAB HEAD: R. Glen Uhrig

PROVIDER: PXD029234 | Pride | 2021-11-03

REPOSITORIES: Pride