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Dietary sugar shifts mitochondrial metabolism and small RNA biogenesis in sperm

ABSTRACT: It is known that exogenous factors influences sperm quality and function, such as toxins, exercise, diet and smoking. Still, the moleuclar mechanisms of this is not understood. Here, we set out to determine how a dietary insult of different sugar levels in the diet affects Drosophila sperm proteome, by dissecting out seminal vesicles containing mature sperm from adult flies. These flies have been on a dietary intervention of two days on a sugar diet of 3, 30 or 300 g/L of sugar. Whole seminal vesicles were then used for mass spectometry to increase our understanding of how an acute dietary intervention may change sperm proteomics.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Sperm, Epithelial Cell, Seminal Vesicle

SUBMITTER: Signe Skog

LAB HEAD: Anita Öst

PROVIDER: PXD029265 | Pride | 2023-07-20

REPOSITORIES: Pride

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			Action	DRS
	D2.msf	Msf
	D2.raw	Raw
	D2_171114.msf	Msf
	D2_171114.raw	Raw
	D2_2_171215.msf	Msf

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Publications

Dietary Sugar Shifts Mitochondrial Metabolism and Small RNA Biogenesis in Sperm.

Ramesh Rashmi R Skog Signe S Örkenby Lovisa L Kugelberg Unn U Nätt Daniel D Öst Anita A

Antioxidants & redox signaling 20230223 16-18

Aims: Increasing concentrations of dietary sugar results in a linear accumulation of triglycerides in male Drosophila, while inducing a U-shaped obesity response in their offspring. Here, using a combination of proteomics and small RNA (sRNA) sequencing, we aimed at understanding the molecular underpinning in sperm for such plasticity. Results: Proteomic analysis of seminal vesicles revealed that increasing concentrations of dietary sugar resulted in a bell-sha ...[more]

PMID: 36509450

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Paternal under and over nutrition modify semen composition and preimplantation embryo developmental kinetics in mice

Project description:Poor parental health can influence the development and eventual health outcomes of their offspring. Many studies have detailed the maternal role yet a father’s health requires further examination as his poor nutritional status has also been shown to have negative consequences on fetal development and impact the long-term health of his offspring. The present study examined how preimplantation embryo development was altered by sub-optimal paternal diet, with specific focus on sperm- and seminal plasma-mediated mechanisms. To address this, male mice were fed a diet to model either under (low protein diet (LPD)) or over (high fat/sugar ‘Western’ diet (WD)) nutrition, LPD or WD supplemented with methyl-donors or a control diet (CD) before mating. Embryo development was assessed using in vitro time-lapse imaging of preimplantation embryos which revealed a significant increase in embryo development rates in all experimental groups when compared to CD embryos. Further analysis of the semen revealed a significant number of differentially expressed seminal plasma proteins in all groups (LPD: 13, MD-LPD: 27, WD: 24, MD-WD: 19) when compared to control. This study highlights a role for paternal nutritional status influencing embryonic development and the maternal reproductive tract via changes to his metabolic and reproductive health. These findings demonstrate potential direct (sperm-mediated) and indirect (seminal plasma-mediated) pathways in which a father's poor diet could shape the long-term health of his offspring

2024-07-25 | PXD044980 | Pride

Mating and sperm free seminal plasma changes gene expression in the oviductal sperm reservoirs of sows

Project description:Sperms being foreign to the female are to be promptly eliminated by the female local immune defense. However, avoiding the local immune defense sperm can be stored for lenthy period in the oviductal sperm reservoir. It is currently unknown whether oviductal sperm reservoirs changes their gene expression to tolerate the spermatozoa after mating or sperm free seminal plasma infusion. Therefore this was tested using Swedish Landrace pigs in this study using cDNA microarray. We used 12 sows seperated into three groups- either oestrus sows were inseminated with 50 ml BTS (control, n=4) or mated with boars (treatment 1, n=4) or inseminated with sperm-free seminal plasma (treatment 2, n=4). The utero-tubal junction was retrieved within 24 h of treatment by operation.

2016-06-23 | E-GEOD-83622 | biostudies-arrayexpress

Murine liver: Sperm vs. seminal plasma influences

Project description:Transcriptional profiling of adult mouse liver tissue comparing offspring derived from sperm and seminal plasma of normal protein diet fed males (controls, NN), sperm and seminal plasma from males fed a low protein diet fed males (LL), sperm from normal protein fed males and seminal plasma from low protein fed males (NL) or sperm from low protein diet fed males and seminal plasma from normal protein diet males (NL). The first letter denotes the diet of the sperm donor and the second letter the diet of the seminal plasma donor. Three-condition experiment: NN vs. LL, NN vs. NL, NN vs. LN. Adult offspring liver tissue. Biological replicates: 7 control (NN), 9 LL, 7 NL and 7 LN. One replicate per array chip.

2022-09-20 | GSE211520 | GEO

Paternal diet defines offspring chromatin state and intergenerational obesity.

Project description:The global rise in obesity has revitalized a search to understand genetic, and in particular, epigenetic factors underlying the disease. We present a Drosophila model of paternal-diet-induced Inter-Generational Metabolic Reprogramming (IGMR) and identify genes required for its encoding in offspring. Intriguingly, we find that as little as two days of dietary intervention in fathers elicits obesity in offspring. Paternal sugar acts as a physiological suppressor of variegation, de-silencing chromatin state-defined transcriptional units in both mature sperm and in offspring embryos. We identify requirements for H3K9/K27me3 dependent reprogramming of metabolic genes in two distinct germline and zygotic windows. Critically, we find evidence that a similar system regulates obesity-susceptibility and phenotype variation in mice and humans. The findings provide insight into the mechanisms underlying intergenerational metabolic reprogramming and carry profound implications for our understanding of phenotypic variation and evolution. RNA-seq on Drosophila embryos and sperm samples fed medium and high sugar.

2014-12-04 | E-GEOD-62668 | biostudies-arrayexpress

Differential proteomics of human seminal fluid Normo Vs Astehnozoospermia

Project description:Scarcely understood defects lead to asthenozoospermia which results in poor fertility outcomes. Incomplete knowledge of these defects hinders the development of new therapies and reliance on interventional therapies, such as in vitro fertilization, increases. Sperm cells, being transcriptionally and translationally silent, necessitate the proteomic approach to study the sperm funnction. We have performed a differential proteomics analysis of human sperm and seminal fluid and identified over 1,700 proteins. We have included 667 proteins in sperm and 430 proteins in seminal plasma dataset for further analysis. Statistical and mathematical analysis combined with pathway analysis and self organizing maps clustering and correlation was performed on the dataset. It was found that sperm proteomic signature combined with statistical analysis as opposed to the seminal plasma proteomic signature can differentiate the normozoospermic versus the asthenozoospermic sperm samples.

2016-12-13 | PXD004098 | Pride

The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts

Project description:Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used whole-cell quantitative proteomics to track the protein composition of sperm across three female reproductive tissues (bursa, seminal receptacle, and spermatheca) and three post-mating timepoints (30 minutes, 2 hours, and 4 days). In combination with an update and expansion of the seminal vesicle sperm proteome and sex-specific isotopic labeling to identify female-contributed sperm proteins, our data provide a comprehensive, quantitative analysis of the molecular life history of Drosophila sperm.

2022-03-14 | PXD027807 | Pride

Quantitation of microRNAs in seminal fluid from men with and without prostate cancer

Project description:The objective of this study was to determine whether prostate cancer-associated miRNAs are present in seminal fluid and, if so, evaluate their diagnostic potential. Small RNA sequencing was used to profile and compare miRNAs in the non-sperm cellular fraction of seminal fluid from men with biopsy-proven cancer (one pooled sample from 6 men) and men with elevated serum PSA but negative biopsy results (one pooled sample from 6 men).

2014-04-15 | E-GEOD-56686 | biostudies-arrayexpress

Stable-protein Pair Analysis as A Novel Strategy to Identify Proteomic Signatures: Application to Seminal Plasma From Infertile Patients.

Project description:Our aim was to define seminal plasma proteome signatures of infertile patients categorized according to their seminal parameters using TMT-LC-MS/MS. To that extent, quantitative proteomic data was analyzed following two complementary strategies: (i) the conventional approach based on standard statistical analyses of relative protein quantification values; and (ii) a novel strategy focused on establishing stable-protein pairs. By conventional analyses, the abundance of some seminal plasma proteins was found to be positively correlated with sperm concentration. However, this correlation was not found for all the peptides within a specific protein, bringing to light the high heterogeneity existing in the seminal plasma proteome due to both the proteolytic fragments and/or the post-translational modifications. This issue was overcome by conducting the novel stable-protein pairs analysis proposed herein. A total of 182 correlations comprising 24 different proteins were identified in the normozoospermic-control population, while this proportion was drastically reduced in infertile patients with altered seminal parameters (18 in patients with reduced sperm motility, 0 in patients with low sperm concentration and 3 in patients with no sperm in the ejaculate). These results suggest the existence of multiple etiologies causing the same alteration in seminal parameters. Additionally, the repetition of the stable-protein pair analysis in the control group by adding the data from a single patient at a time enabled to identify alterations in the stable-protein pairs profile of individual patients with altered seminal parameters. These results suggest potential underlying pathogenic mechanisms in individual infertile patients, and might open up a window to its application in the personalized diagnostic of male infertility.

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Project description:Sperm competition theory predicts that males should tailor ejaculates according to their social status. Here we test this in a model vertebrate, the house mouse (Mus musculus domesticus), combining experimental data with a quantitative proteomics analysis of seminal fluid composition. Our analyses reveal that both sperm production and the relative production of proteins found in seminal fluid differ according to social dominance. Notably, whereas dominant males produce and ejaculate more sperm, subordinate males produce greater relative amounts of key proteins used in the formation of copulatory plugs. These findings have important implications for understanding the dynamics and outcome of sperm competition.

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Project description:Seminal plasma contributes to the safe environment for sperm maturation, sperm viability and fertilization in mammals (Kumar 2013). Moreover, seminal plasma is a promising source for the study of potential reproductive biomarkers, because it is a complex mixture of secretions from testis, epididymis and male accessory sex glands (González-Cadavid et al. 2014). The protein composition of mammalian seminal plasma varies among species, and has important effects on sperm function (Mortarino et al. 1998). In rabbits, seminal plasma has a positive effect in maintaining sperm motility and viability during in vitro storage (Castellini et al. 2000). To study proteins on a large scale, the application of a proteomic approach is mandatory (Dietrich 2014). Rabbit seminal plasma proteome has been proved different between rabbit genetic line A (maternal) and R (growth) (Viudes-de-Castro et al. (2004), Safaa et al. (2008), Casares-Crespo 2016) and between seasons (Casares-Crespo 2016). In these previous studies a traditional 1 dimension polyacrylamide gel was done, identifying only major proteins visible after Coomassie Colloidal Blue staining and obtaining the relative quantity of these protein bands. On the contrary, new approaches such as liquid chromatography in-gel or in-solution digestion coupled with mass spectrometric analysis are a powerful, yet relatively uncomplicated strategy to identify and characterize proteins. Mass spectrometry-based proteomics has become the tool of choice for identifying and quantifying the proteome of an organism (Karpievitch 2010). Therefore, the present study was conducted to describe the seminal plasma proteome of rabbits, compare the differentially expressed seminal plasma proteins between genetic lines A and R and study the effect of season in rabbit seminal plasma proteome.

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