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Exploring the mechanism of action of Canmei formula against colorectal adenoma through multi-omics technique

ABSTRACT: Background: Canmei formula (CMF) is a traditional Chinese medicine compound with definite effect on the prevention and treatment of colorectal adenoma (CRA). It can prevent the transformation of intestinal inflammation to cancer. This study explored the mechanism of action of CMF in anti-CRA using multi-omics techniques. Method: Mice were randomly divided into 4 groups: blank group (Control), high-fat diet (HFD) + AOM/DSS colorectal adenoma model (ADH) groups, Canmei formula treatment group (ADH-CMF) and sulfasalazine treatment group (Sul). Except for the blank group, ADH model was established in the other 3 groups by intraperitoneal injection with AOM reagent, and then mice was given 2.5%DSS in free drinking water and high-fat diet. The mice in the blank group and ADH group were intragastrically perfused with normal saline, and the mice in the other 2 groups were treated with corresponding drugs for 20 weeks. During this period, the changes of physical signs of mice in each group were observed. The differentially expressed genes and proteins in the Control group, ADH group and ADH-CMF group were detected by RNA-seq transcriptome sequencing and Tandem Mass Tags (TMT) quantitative proteomics. After the combined analysis and verification. The key targets were analyzed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Results : A total of 2548 differential genes were obtained by transcriptomics analysis, and 45 differential proteins were obtained by proteomics analysis. The results of proteomics data and experimental verification showed that CMF mainly acted on the Phospholysine Phosphohistidine Inorganic Pyrophosphate Phosphatase (LHPP) target. GO analysis showed that the targets of CMF were involved in the biological processes such as cellular process, metabolic process and biological regulation. KEGG analysis showed that those genes were involved in oxidative phosphorylation, cell senescence, and Metabolic pathways. Studies have shown that Lhpp overexpression of Lhpp impeded Colorectal cancer cell growth and proliferation in vitro, and was associated with a change in PI3K/AKT activity.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Colon

DISEASE(S): Stage Iv Colorectal Cancer

SUBMITTER: xiaoling fu

LAB HEAD: National Pharmaceutical Engineering Research Cente

PROVIDER: PXD029296 | Pride | 2022-02-16

REPOSITORIES: Pride

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			Action	DRS
	20200220_TMT_10_120min_Cl17FXL_bjhb1_1.raw	Raw
	20200220_TMT_10_120min_Cl17FXL_bjhb1_10.raw	Raw
	20200220_TMT_10_120min_Cl17FXL_bjhb1_2.raw	Raw
	20200220_TMT_10_120min_Cl17FXL_bjhb1_3.raw	Raw
	20200220_TMT_10_120min_Cl17FXL_bjhb1_4.raw	Raw

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Exploring the Mechanism of Action of Canmei Formula Against Colorectal Adenoma Through Multi-Omics Technique.

Guo Cui C Liu Xiaoqiang X Xu Yimin Y Han Xinyue X Xie Runnan R Meng Xiangxue X Li Yuan Y Chen Tongyu T Cheng Zhihong Z Fu Xiaoling X

Frontiers in cell and developmental biology 20211129

<b>Background:</b> Canmei formula (CMF) is a traditional Chinese medicine compound with definite effect on the prevention and treatment of colorectal adenoma (CRA). CMF can prevent the transformation of intestinal inflammation to cancer. This study explored the mechanism of action of CMF in anti-CRA using multi-omics techniques. <b>Method:</b> The mice were randomly divided into four groups: blank group (Control), high-fat diet (HFD) + AOM/DSS colorectal adenoma model (ADH) groups, Canmei formul ...[more]

PMID: 34926462

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