Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: Olga T. Schubert
LAB HEAD: Leonid Kruglyak
PROVIDER: PXD029363 | Pride | 2022-11-07
REPOSITORIES: Pride
Action | DRS | |||
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IRA2_A.raw | Raw | |||
IRA2_B.raw | Raw | |||
IRA2_C.raw | Raw | |||
POP1_A.raw | Raw | |||
POP1_B.raw | Raw |
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eLife 20221103
Proteins are key molecular players in a cell, and their abundance is extensively regulated not just at the level of gene expression but also post-transcriptionally. Here, we describe a genetic screen in yeast that enables systematic characterization of how protein abundance regulation is encoded in the genome. The screen combines a CRISPR/Cas9 base editor to introduce point mutations with fluorescent tagging of endogenous proteins to facilitate a flow-cytometric readout. We first benchmarked bas ...[more]