Dataset Information

Aging-Associated Changes in CSF Protein Abundances and Structures Revealed by a Modified LiP-MS Screen

ABSTRACT: We performed LiP-MS on CSF from young and old mice with a modified analysis pipeline. We found 38 protein groups that change in abundance with aging, most dominantly immunoglobulins of the IgM subclass. We discovered six high-confidence candidates that appeared to change in structure with aging.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: Steven Shuken

LAB HEAD: Tony Wyss-Coray

PROVIDER: PXD031174 | Pride | 2022-08-22

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20200210_SShuken_WyssCoray_11036_C1C.raw	Raw
	20200210_SShuken_WyssCoray_11036_C1P.raw	Raw
	20200210_SShuken_WyssCoray_11036_C2C.raw	Raw
	20200210_SShuken_WyssCoray_11036_C2P.raw	Raw
	20200210_SShuken_WyssCoray_11036_C3C.raw	Raw

Items per page:

1 - 5 of 89

Similar Datasets

Project description:Mutations in nuclear genes encoding for mitochondrial proteins very long-chain acyl-CoA dehydrogenase (VLCAD) and trifunctional protein (TFP) cause rare autosomal recessive disorders. Studies in fibroblasts derived from patients with mutations in VLCAD and TFP exhibit mitochondrial defects. To gain insights on pathological changes that account for the mitochondria l deficits we performed quantitative proteomic, biochemical, and morphometric analyses in fibroblasts derived from subjects with three different VLCAD and three different TFP mutations. Proteomic data that was corroborated by antibody-based detection, indicated reduced levels of VLCAD and TFP protein in cells with VLCAD and TFP mutations respectively, which in part accounted for the diminished fatty acid oxidation capacity. Decreased mitochondrial respiratory capacity in cells with VLCAD and TFP mutations was quantified after glucose removal and cells with TFP mutations had lower levels of glycogen. Despite these energetic deficiencies, the cells with VLCAD and TFP mutations did not exhibit changes in mitochondria morphology, distribution, fusion and fission, quantified by either confocal or transmission electron microscopy and corroborated by proteomic and antibody-based protein analysis. Fibroblasts with VLCAD and to a lesser extend cells with TFP mutations had increased levels of mitochondrial respiratory chain proteins and proteins that facilitate the assembly of respiratory complexes. With the exception of reduced levels of catalase and glutathione S-transferase theta-1 in cells with TFP mutations, the levels of 45 proteins across all major intracellular antioxidant networks were similar between cells with VLCAD and TFP mutations and non-disease controls. Collectively the data indicate that despite the metabolic deficits, cells with VLCAD and TFP mutations maintain their proteomic integrity to preserve cellular and mitochondria architecture, support energy production and protect against oxidative stress.

Dataset Information

Aging-Associated Changes in CSF Protein Abundances and Structures Revealed by a Modified LiP-MS Screen

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets