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Rat parathyroid glands LC-MS/MS

ABSTRACT: The mechanisms of chronic kidney disease-associated secondary hyperparathyroidism are partially understood. In this project we aimed to gain new information and propose research hypotheses by proteome and phosphoproteome profiling of normal and hyperplastic rat parathyroid glands. Glands were microdissected from rats on normal control diet or CKD-inducing high adenine diet enriched with phosphorus to produce secondary hyperparathyroidism. Protein extracts were pooled from 4 glands (2 rats) for proteome profiling (3 normal and 3 CKD pools), and from 12 glands (6 rats) for phosphoproteome profiling (2 normal and 2 CKD pools).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Parathyroid Gland

DISEASE(S): Chronic Kidney Disease

SUBMITTER: Iddo Ben-Dov

LAB HEAD: Iddo Zeev Ben-Dov

PROVIDER: PXD029368 | Pride | 2022-06-28

REPOSITORIES: Pride

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			Action	DRS
	Seq58185-6_QE2.raw	Raw
	Seq58187-8_QE2.raw	Raw
	Seq58189-90_QE2.raw	Raw
	Seq58191-2_QE2.raw	Raw
	Seq58193-4_QE2.raw	Raw

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Rat parathyroid phosphoproteins LC-MS/MS

Project description:The mechanisms of chronic kidney disease-associated secondary hyperparathyroidism are partially understood. In this project we aimed to gain new information and propose research hypotheses by proteome and phosphoproteome profiling of normal and hyperplastic rat parathyroid glands. Glands were microdissected from rats on normal control diet or CKD-inducing high adenine diet enriched with phosphorus to produce secondary hyperparathyroidism. Protein extracts were pooled from 12 glands (6 rats) for phosphoproteome profiling (3 normal and 3 CKD pools).

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