Dataset Information

Analysis of protein secretome of human hepatocellular carcinoma (HCC) Cancer Associated Fibroblasts (CAFs) after long term (14 days) treatment with Transforming Growth Factor beta 1 (TGF beta 1)

ABSTRACT: Cancer-Associated Fibroblasts (CAFs) were isolated from specimens taken from primary human hepatocellular carcinoma (HCC) nodules (4 patients in total), characterized for expression of typical stromal markers (including vimentin and alpha smooth muscle actin), and then incubated in complete medium (Iscove's Modified Dulbecco Medium, IMDM + 20% fetal bovine serum + antibiotic/antimycotic) without any treatment, or in the presence of Transforming Growth Factor Beta 1 (TGF beta 1) at a concentration of 5 ng/ml. The medium and the treatment were renewed every 2 days until the 14th day. At that time point, cells were washed 4 times with serum-free IMDM medium and then incubated with serum free IMDM medium for 48 hours to allow for enrichment with secreted CAFs proteins. The CAFs conditioned medium was then collected, concentrated using a centricon device (10 kDa cutoff), assayed for protein concentration, and finally subjected to Liquid Chromatography with tandem mass spectrometry (LC-MS/MS).

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver, Fibroblast

DISEASE(S): Hepatocellular Carcinoma

SUBMITTER: Francesco Dituri

LAB HEAD: Francesco Dituri

PROVIDER: PXD029586 | Pride | 2021-11-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MSB-7943_Bari_Dituri_080619.sf3	Other
	MSB-8870_Bari_Dituri_100720.sf3	Other
	MSB42116Band_01.raw	Raw
	MSB42116Band_02.raw	Raw
	MSB42116Band_03.raw	Raw

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Dataset Information

Analysis of protein secretome of human hepatocellular carcinoma (HCC) Cancer Associated Fibroblasts (CAFs) after long term (14 days) treatment with Transforming Growth Factor beta 1 (TGF beta 1)

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