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Protein-protein interactions of BRG1 or BRG1-HSA in SW13 cells

ABSTRACT: The SWI/SNF complex remodels chromatin in an ATP-dependent manner through the ATPase subunits BRG1 and BRM. Chromatin remodeling alters nucleosome structure to change gene expression, however aberrant remodeling and gene expression can result in cancer. The function and localization on chromatin of the SWI/SNF complex depends on the protein makeup of the complex. Here we report the protein-protein interactions of wild-type BRG1 or mutant BRG1 in which the HSA domain has been deleted (BRG1-HSA). We demonstrate the interaction of BRG1 with most SWI/SNF complex members and a failure of a number of these members to interact with BRG1-HSA. These results demonstrate that the HSA domain of BRG1 is a critical interaction platform for the correct formation of SWI/SNF remodeling complexes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Jason Williams

LAB HEAD: Jason Grant Williams

PROVIDER: PXD029647 | Pride | 2023-02-15

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Dietrich_021120.xlsx	Xlsx
	Dietrich_BRG1_IP_031519_excel.xlsx	Xlsx
	Dietrich_HSA_IP_031519_excel.xlsx	Xlsx
	QE1020520_Dietrich_01_HSA_IgG_A.mgf	Mgf
	QE1020520_Dietrich_01_HSA_IgG_A.raw	Raw

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