Dataset Information

IP-MS of GFP tagged MRV μ2 protein

ABSTRACT: To determine which cellular proteins interacts with the viral MRV protein μ2 and its mutant μ2-P208S, GFP-tagged constructions of μ2 and μ2-P208S in both C- and N- terminus were transfected in 293T cells alongside control GFP alone for 24h, and submitted to GFP pulldown before IP were prepared for MS analysis.

INSTRUMENT(S): Bruker Daltonics timsTOF series

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Early Embryonic Cell

SUBMITTER: Simon Boudreault

LAB HEAD: Martin Bisaillon

PROVIDER: PXD029701 | Pride | 2022-06-09

REPOSITORIES: Pride

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Publications

Reovirus μ2 protein modulates host cell alternative splicing by reducing protein levels of U5 snRNP core components.

Boudreault Simon S Durand Mathieu M Martineau Carole-Anne CA Perreault Jean-Pierre JP Lemay Guy G Bisaillon Martin M

Nucleic acids research 20220501 9

Mammalian orthoreovirus (MRV) is a double-stranded RNA virus from the Reoviridae family presenting a promising activity as an oncolytic virus. Recent studies have underlined MRV's ability to alter cellular alternative splicing (AS) during infection, with a limited understanding of the mechanisms at play. In this study, we investigated how MRV modulates AS. Using a combination of cell biology and reverse genetics experiments, we demonstrated that the M1 gene segment, encoding the μ2 protein, is t ...[more]

PMID: 35489070

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			Action	DRS
	1.IP_pEGFPN1_1.rar	Other
	1.IP_pEGFPN1_2.rar	Other
	1.IP_pEGFPN1_3.rar	Other
	2.pEGFPN1_Mu2_1.rar	Other
	2.pEGFPN1_Mu2_2.rar	Other